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PMID: 2468162 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Brain phospholipase C isozymes: differential mRNA localizations by in situ hybridization.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 86 ·No. 8 ·1989-04-00 ·Pages 2923-7

Ross CA, MacCumber MW, Glatt CE, Snyder SH

Abstract

mRNAs for isozymes of phospholipase C (PLC) were localized in rat brain by in situ hybridization with oligonucleotide probes for PLC isozymes I, II, and III of Rhee's group [Suh, P.-G., Ryu, S. H., Moon, K. H., Suh, H. W. & Rhee, S. G. (1988) Proc. Natl. Acad. Sci. USA 85, 5419-5423 and (1988) Cell 54, 161-169], and isozyme I of Bennett and Crooke [Bennett, C. F., Balcarek, J. M., Varrichio, A. & Crooke, S. T. (1988) Nature (London) 334, 268-270], which we designate PLC-A. The isozymes displayed different localizations. PLC-A mRNA was highest in the mitral cell layer of the olfactory bulb, choroid plexus, hippocampus and dentate gyrus, magnocellular hypothalamic nuclei, rostral raphe nuclei, and cerebellar Purkinje cells. PLC-I was highest in the internal granular cell layer of the olfactory bulb, cerebral cortex, caudate, nucleus of the lateral olfactory tract, reticular nucleus of thalamus, hippocampus and dentate gyrus, and granule cell layer of the cerebellum. PLC-II had a more widespread distribution, with relatively high levels in the internal granular layer of the olfactory bulb, hippocampus and dentate gyrus, and cerebellar Purkinje and granule cells. PLC-III label was low throughout the brain. These distributions suggest selective coupling of individual PLC isozymes with particular postsynaptic receptors. PLC-A may be preferentially associated with 5-hydroxytryptamine 1C receptors, vasopressin V1 receptors, and a subtype of glutamate receptors. PLC-I may be linked to muscarinic m1 and m3 receptors as well as other receptors. The distribution of PLC-II mRNA resembles that of src protooncogene, with which it displays sequence homology.

MeSH Terms
Animals Brain/enzymology Isoenzymes/genetics Nucleic Acid Hybridization Phosphatidylinositols/physiology RNA RNA Probes RNA, Antisense RNA, Messenger/metabolism Rats/embryology Type C Phospholipases/genetics
Chemicals
Isoenzymes Phosphatidylinositols RNA Probes RNA, Antisense RNA, Messenger RNA Type C Phospholipases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Ross C A
Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205.
MacCumber M W
Glatt C E
Snyder S H
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.86.8.2923
Published
1989-04-00
Pages
2923-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC287032
Subset
IM
Grants
NIDA NIH HHS · DA-00074 · United States
NIDA NIH HHS · DA-00266 · United States
NIMH NIH HHS · MH-18501 · United States
External Links
PubMed source DOI: 10.1073/pnas.86.8.2923 PMC Full Text View journal details
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