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PMID: 2459129 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Isolation, sequence, and differential expression of a human K7 gene in simple epithelial cells.

The Journal of cell biology ·Vol. 107 ·No. 4 ·1988-10-00 ·Pages 1337-50

Glass C, Fuchs E

Abstract

Simple epithelial cells synthesize a different set of keratins than epidermal cells. In experiments reported in this manuscript, we show that the base level of keratin expression in simple epithelial cells is variable for different cell types, and that, in some simple epithelia, this level can be upregulated by increasing the exposure of cells to retinoids, but not glucocorticoids or estradiol. To elucidate the molecular mechanisms underlying simple epithelial keratin gene regulation, we have isolated and characterized a human gene encoding the simple epithelial keratin K7. By examining the possible regulatory elements of this gene and by investigating the behavior of this gene introduced transiently into simple epithelial cells, we have uncovered a possible basis for the differential expression of epidermal and simple epithelial keratin genes.

MeSH Terms
Amino Acid Sequence Base Sequence Blotting, Southern Cell Line Cloning, Molecular Electrophoresis, Gel, Two-Dimensional Epithelium/physiology Estrogens/pharmacology Fluorescent Antibody Technique Gene Expression Regulation/drug effects Genes Glucocorticoids/pharmacology Keratins/genetics Molecular Sequence Data Regulatory Sequences, Nucleic Acid Transcription, Genetic Tretinoin/pharmacology
Chemicals
Estrogens Glucocorticoids Tretinoin Keratins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Glass C
Department of Molecular Genetics, University of Chicago, IL 60637.
Fuchs E
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59 references, click to expand
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.107.4.1337
Published
1988-10-00
Pages
1337-50
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2115265
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.107.4.1337 PMC Full Text View journal details
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