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PMID: 2580298 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Complete sequence of a gene encoding a human type I keratin: sequences homologous to enhancer elements in the regulatory region of the gene.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 82 ·No. 6 ·1985-03-00 ·Pages 1609-13

Marchuk D, McCrohon S, Fuchs E

Abstract

We report here the complete nucleotide sequence of a gene encoding the 50-kDa keratin expressed in abundance in human epidermal cells. According to its sequence, this gene has a single transcriptional initiation site and a single polyadenylylation signal. Nuclease S1 mapping of this gene with total human epidermal mRNA confirmed the presence of a single initiation site for the 50-kDa keratin gene. When the regulatory sequences 5' upstream from this gene were examined, three sequences that share significant homology with viral and immunoglobulin enhancer elements were found. In comparison, the sequence of the regulatory region of vimentin, a structurally similar intermediate filament gene, was highly divergent [Quax, W., Egberts, W. V., Hendriks, W., Quax-Jeuken, Y. & Bloemendal, H. (1983) Cell 35, 215-223]. This finding may provide a clue to understanding the molecular mechanisms underlying the widely varying levels of expression of different intermediate filament genes in different tissues.

MeSH Terms
Base Sequence Enhancer Elements, Genetic Gene Expression Regulation Genes, Regulator Humans Keratins/genetics Protein Biosynthesis Transcription, Genetic Vimentin/genetics
Chemicals
Vimentin Keratins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Marchuk D
McCrohon S
Fuchs E
References (33)
33 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.82.6.1609
Published
1985-03-00
Pages
1609-13
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC397321
Subset
IM
Databases
GENBANK
J00124
External Links
PubMed source DOI: 10.1073/pnas.82.6.1609 PMC Full Text View journal details
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