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PMID: 2867473 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Primary structure and expression of a functional human glucocorticoid receptor cDNA.

Nature ·Vol. 318 ·No. 6047 ·1985-00-19 ·Pages 635-41

Hollenberg SM, Weinberger C, Ong ES, Cerelli G, Oro A, Lebo R, Thompson EB, Rosenfeld MG, Evans RM

Abstract

Identification of complementary DNAs encoding the human glucocorticoid receptor predicts two protein forms, of 777 (alpha) and 742 (beta) amino acids, which differ at their carboxy termini. The proteins contain a cysteine/lysine/arginine-rich region which may define the DNA-binding domain. Pure radiolabelled glucocorticoid receptor, synthesized in vitro, is immunoreactive and possesses intrinsic steroid-binding activity characteristic of the native glucocorticoid receptor.

MeSH Terms
Amino Acid Sequence Base Sequence DNA/analysis Electrophoresis, Polyacrylamide Gel Gene Expression Regulation Humans Macromolecular Substances Poly A/analysis RNA, Messenger/analysis Receptors, Glucocorticoid/genetics
Chemicals
Macromolecular Substances RNA, Messenger Receptors, Glucocorticoid Poly A DNA
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Hollenberg S M
Weinberger C
Ong E S
Cerelli G
Oro A
Lebo R
Thompson E B
Rosenfeld M G
Evans R M
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Article Info
Journal
Nature
Abbr.
Nature
ISSN
0028-0836
DOI
10.1038/318635a0
Published
1985-00-19
Pages
635-41
Language
English
Region
England
NLM ID
0410462
PMCID
PMC6165583
Subset
IM
Grants
Howard Hughes Medical Institute · United States
Databases
GENBANK
M10901, M11050
External Links
PubMed source DOI: 10.1038/318635a0 PMC Full Text View journal details
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