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PMID: 2446013 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Transcription of interferon-stimulated genes is induced by adenovirus particles but is suppressed by E1A gene products.

Journal of virology ·Vol. 62 ·No. 1 ·1988-01-00 ·Pages 114-9

Reich N, Pine R, Levy D, Darnell JE

Abstract

Interferon treatment of cell cultures results in the rapid transcriptional induction of a specific set of genes. In this paper we explore the effect of cellular infection by several adenoviruses, both wild type and mutant, on the expression of these genes. Infection with adenovirus induces the transcription of the interferon-stimulated genes in the absence of any protein synthesis. In fact, the inhibition of protein synthesis during a wild-type infection produces enhanced stimulation of transcription of these genes. Experiments with viral mutants indicate the ability to specifically suppress this transcription maps to the E1A gene. In addition, the E1A gene products are capable of suppressing the specific transcriptional induction of interferon-stimulated promoters during cotransfection experiments and therefore presumably during viral infection. The dual effect of adenovirus on the expression of interferon-stimulated genes may represent an example of action and evolutionary reaction between virus and host.

MeSH Terms
Adenovirus Early Proteins Adenoviruses, Human/genetics Cell Line Gene Expression Regulation Interferons/physiology Oncogene Proteins, Viral/physiology Protein Biosynthesis RNA, Messenger/genetics RNA, Viral/genetics Time Factors Transcription, Genetic Transfection
Chemicals
Adenovirus Early Proteins Oncogene Proteins, Viral RNA, Messenger RNA, Viral Interferons
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Reich N
Rockefeller University, New York, New York 10021-6399.
Pine R
Levy D
Darnell J E
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44 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.62.1.114-119.1988
Published
1988-01-00
Pages
114-9
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC250508
Subset
IM
Grants
NCI NIH HHS · CA 16006-14 · United States
NCI NIH HHS · CA 18213-11 · United States
External Links
PubMed source DOI: 10.1128/JVI.62.1.114-119.1988 PMC Full Text View journal details
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