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PMID: 24429668 Published · ppublish English Journal Article

Transport of proteins into chloroplasts.

Photosynthesis research ·Vol. 17 ·No. 1-2 ·1988-07-00 ·Pages 173-94

Lubben TH, Theg SM, Keegstra K

Abstract

The import of cytoplasmically synthesized proteins into chloroplasts involves an interaction between at least two components; the precursor protein, and the import apparatus in the chloroplast envelope membrane. This review summarizes the information available about each of these components. Precursor proteins consist of an amino terminal transit peptide attached to a passenger protein. Transit peptides from various precurosrs are diverse with respect to length and amino acid sequence; analysis of their sequences has not revealed insight into their mode of action. A variety of foreign passenger proteins can be imported into chloroplasts when a transit peptide is present at the amino terminus. However, foreign passenger proteins are not imported as efficiently as natural passenger proteins, and some chimeric precursor proteins are not imported into chloroplasts at all. Therefore, the passenger protein, as well as the transit peptide, influences the import process. Import begins by binding of the precursor to the chloroplast surface. It has been suggested that this binding is mediated by a receptor, but evidence to support this hypothesis remains incomplete and a receptor protein has not yet been characterized. Protein translocation requires energy derived from ATP hydrolysis, although there are conflicting reports as to where hydrolysis occurs and it is unclear how this energy is utilized. The mechanism(s) whereby proteins are translocated across either the two envelope membranes or the thylakoid membrane is not known.

Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Lubben T H
Botany Department, University of Wisconsin, 53706, Madison, Wisconsin, USA.
Theg S M
Keegstra K
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Article Info
Journal
Photosynthesis research
Abbr.
Photosynth Res
ISSN
0166-8595
DOI
10.1007/BF00047688
Published
1988-07-00
Pages
173-94
Language
English
Region
Netherlands
NLM ID
100954728
External Links
PubMed source DOI: 10.1007/BF00047688 View journal details
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