Double infection of tobacco plants by two complementing octopine T-region mutants of Agrobacterium tumefaciens.

Plant molecular biology ·Vol. 1 ·No. 3 ·1982-09-00 ·Pages 217-26

Ooms G, Molendijk L, Schilperoort RA

Abstract

The molecular basis of complementation by a mixture of two different types of octopine T-region mutants (LBA4060 and LBA4210) was studied. Six randomly chosen cellular clones derived from a tumor obtained after mixed infection were analyzed for their T-DNA content via Southern blot hybridization. The clones appeared to contain T-DNA that originated from each of both mutants, indicating that they developed from doubly infected single cells. Genetic complementation, therefore, might explain at least in part the observed complementation phenomenon. However, complementation as a result of cross-feeding between separately transformed cells could not be excluded. Following protoplast isolation, small aggregates might have formed that developed into the clones analyzed.

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Ooms G

Department of Biochemistry, State University of Leiden, Wassenaarseweg 64, 2333 AL, Leiden, The Netherlands.

Molendijk L

Schilperoort R A

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Article Info

Journal

Plant molecular biology

Abbr.

Plant Mol Biol

ISSN

0167-4412

DOI

10.1007/BF00021033

Published

1982-09-00

Pages

217-26

Language

English

Region

Netherlands

NLM ID

9106343

External Links

PubMed source DOI: 10.1007/BF00021033 View journal details

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Double infection of tobacco plants by two complementing octopine T-region mutants of Agrobacterium tumefaciens.

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