The expression of tumour markers in intraspecific somatic hybrids of normal and crown gall cells from Nicotiana tabacum.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik ·Vol. 56 ·No. 5 ·1980-09-00 ·Pages 203-8

Wullems GJ, Molendijk L, Schilperoort RA

Abstract

Following fusion of protoplasts from crown gall tumour calli, characterized by hormone independent growth, and protoplasts from normal tissues of a streptomycin-resistant mutant, SR1, we selected hormone independent streptomycin-resistant calli in Nicotiana tabacum. The tumour line, B6S3, lost the ability to form shoots. Some of the selected lines, similar to SR1, however, are morphogenic. Both calli and shoots contained the tumour specific enzyme lysopinedehydrogenase. The hybrid shoots are resistant to Agrobacterium infection and do not root. These tumorous properties are dominantly expressed in the somatic hybrids.

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Wullems G J

Department of Biochemistry, State University of Leiden, Leiden, the Netherlands.

Molendijk L

Schilperoort R A

References (13)

13 references, click to expand

Haploid plants from pollen grains.
Science. 1969 Jan 3;163(3862):85-7 PMID: 17780179
Cell walls of crown-gall tumors and embryonic plant tissues lack agrobacterium adherence sites.
Science. 1978 Mar 10;199(4333):1075-8 PMID: 17844419
Plant tumors.
Biochim Biophys Acta. 1978 Oct 27;516(2):167-91 PMID: 365239
Supercoiled circular DNA in crown-gall inducing Agrobacterium strains.
J Mol Biol. 1974 Jun 15;86(1):109-27 PMID: 4854526
A rapid micro scale method for the detection of lysopine and nopaline dehydrogenase activities.
Biochim Biophys Acta. 1978 Dec 8;527(2):497-500 PMID: 31918
Stable incorporation of plasmid DNA into higher plant cells: the molecular basis of crown gall tumorigenesis.
Cell. 1977 Jun;11(2):263-71 PMID: 890735
Octopine and nopaline synthesis and breakdown genetically controlled by a plasmid of Agrobacterium tumefaciens.
Mol Gen Genet. 1976 May 7;145(2):177-81 PMID: 934053
Streptomycin-resistant plants from callus culture of haploid tobacco.
Nat New Biol. 1973 Jul 4;244(131):29-30 PMID: 4515911
Coordinated regulation of octopine degradation and conjugative transfer of Ti plasmids in Agrobacterium tumefaciens: evidence for a common regulatory gene and separate operons.
J Bacteriol. 1978 Nov;136(2):775-85 PMID: 711678
The effect of high pH and calcium on tobacco leaf protoplast fusion.
Z Naturforsch C. 1973 Nov-Dec;28(11):737-41 PMID: 4273693
Non-Mendelian streptomycin-resistant tobacco mutant with altered chlorplasts and mitochondria.
Nature. 1975 May 29;255(5507):401-2 PMID: 1128700
Genetic map of an octopine TI-plasmid.
Plasmid. 1979 Jul;2(3):347-57 PMID: 482427
A method for high-frequency intergeneric fusion of plant protoplasts.
Planta. 1974 Dec;115(4):355-67 PMID: 24458930

Article Info

Journal

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik

Abbr.

Theor Appl Genet

ISSN

0040-5752

DOI

10.1007/BF00295450

Published

1980-09-00

Pages

203-8

Language

English

Region

Germany

NLM ID

0145600

External Links

PubMed source DOI: 10.1007/BF00295450 View journal details

Analysis Services

The expression of tumour markers in intraspecific somatic hybrids of normal and crown gall cells from Nicotiana tabacum.

Contact

WeChat Official Account