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PMID: 24276797 Published · ppublish English Journal Article

T-DNA rearrangements due to tissue culture: somaclonal variation in crown gall tissues.

Plant molecular biology ·Vol. 9 ·No. 1 ·1987-01-00 ·Pages 51-7

Peerbolte R, Ruigrok P, Wullems G, Schilperoort R

Abstract

After three years of apparent stability in tissue culture, the single cell derived shooty crown gall line sNT1.013 produced a revertant shoot which had switched from non-rooting (Rod(+)) and octopine synthesizing (Ocs(+)) to Rod(-) Ocs(-), indicating that in this revertant TL-DNA genes 4 (causing the Rod(+) trait) and gene 3 (causing the Ocs(+) trait) had been inactivated. Southern blots revealed that the inactivation of these T-DNA genes was the result of a considerable rearrangement of DNA sequences, accompanied by deletions and possibly also by DNA amplifications. This study for the first time unambiguously proves that foreign genes which have been introduced via Agrobacterium tumefaciens can, at a low frequency, be inactivated after T-DNA integration because of reorganization of T-DNA sequences during tissue culture. This can be considered as an event of somaclonal variation.

Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Peerbolte R
MolBas Research Group, Dept. of Plant Molecular Biology, Leiden University, Wassenaarseweg 64, 2333 AL, Leiden, The Netherlands.
Ruigrok P
Wullems G
Schilperoort R
References (23)
23 references, click to expand
  1. A rapid micro scale method for the detection of lysopine and nopaline dehydrogenase activities.
    Biochim Biophys Acta. 1978 Dec 8;527(2):497-500 PMID: 31918
  2. Changes in T-DNA methylation and expression are associated with phenotypic variation and plant regeneration in a crown gall tumor line.
    Mol Gen Genet. 1984;197(3):437-46 PMID: 6084805
  3. Clones from a shooty tobacco crown gall tumor II: irregular T-DNA structures and organization, T-DNA methylation and conditional expression of opine genes.
    Plant Mol Biol. 1986 Jul;7(4):285-99 PMID: 24302370
  4. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.
    J Mol Biol. 1977 Jun 15;113(1):237-51 PMID: 881736
  5. Coordinated regulation of octopine degradation and conjugative transfer of Ti plasmids in Agrobacterium tumefaciens: evidence for a common regulatory gene and separate operons.
    J Bacteriol. 1978 Nov;136(2):775-85 PMID: 711678
  6. Genetic identification of functions of TR-DNA transcripts in octopine crown galls.
    EMBO J. 1984 Jan;3(1):141-6 PMID: 16453491
  7. Clones from a shooty tobacco crown gall tumor I: deletions, rearrangements and amplifications resulting in irregular T-DNA structures and organizations.
    Plant Mol Biol. 1986 Jul;7(4):265-84 PMID: 24302369
  8. Retention of tumor markers in F1 progeny plants from in vitro induced octopine and nopaline tumor tissues.
    Cell. 1981 Jun;24(3):719-27 PMID: 7249079
  9. Restriction endonuclease mapping of the octopine tumor-inducing plasmid pTiAch5 of Agrobacterium tumefaciens.
    Plasmid. 1981 Sep;6(2):249-53 PMID: 6272339
  10. Stability and expression of transferred DNA in F1 tobacco transformants studied at various states of differentiation.
    Planta. 1987 Dec;172(4):448-62 PMID: 24226063
  11. Transformed cell clones as a tool to study T-DNA integration mediated by Agrobacterium tumefaciens.
    J Mol Biol. 1986 Mar 20;188(2):129-45 PMID: 3723593
  12. T-DNA organization in homogeneous and heterogeneous octopine-type crown gall tissues of Nicotiana tabacum.
    Cell. 1982 Sep;30(2):589-97 PMID: 6291777
  13. Nucleotide sequence of the T-DNA region from theA grobacterium tumefaciens octopine Ti plasmid pTi15955.
    Plant Mol Biol. 1983 Nov;2(6):335-50 PMID: 24318453
  14. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  15. Mendelian transmission of genes introduced into plants by the Ti plasmids of Agrobacterium tumefaciens.
    Mol Gen Genet. 1981;183(2):209-13 PMID: 6948997
  16. The TL-DNA in octopine crown-gall tumours codes for seven well-defined polyadenylated transcripts.
    EMBO J. 1982;1(1):139-46 PMID: 16453403
  17. Foreign DNA sequences in crown gall teratomas and their fate during the loss of the tumorous traits.
    Mol Gen Genet. 1980;177(4):707-14 PMID: 6929944
  18. Single gene mutations in tomato plants regenerated from tissue culture.
    Science. 1983 Sep 2;221(4614):949-51 PMID: 17748463
  19. A simple and general method for transferring genes into plants.
    Science. 1985 Mar 8;227(4691):1229-31 PMID: 17757866
  20. Structure, organization and expression of transferred DNA in Nicotiana plumbaginifolia crown-gall tissues.
    Planta. 1987 Jul;171(3):393-405 PMID: 24227439
  21. Streptomycin-resistant plants from callus culture of haploid tobacco.
    Nat New Biol. 1973 Jul 4;244(131):29-30 PMID: 4515911
  22. Internal organization, boundaries and integration of Ti-plasmid DNA in nopaline grown gall tumours.
    J Mol Biol. 1980 Dec 15;144(3):353-76 PMID: 7253020
  23. Revertant seedlings from crown gall tumors retain a portion of the bacterial Ti plasmid DNA sequences.
    Proc Natl Acad Sci U S A. 1981 Jul;78(7):4151-5 PMID: 16593055
Article Info
Journal
Plant molecular biology
Abbr.
Plant Mol Biol
ISSN
0167-4412
DOI
10.1007/BF00017986
Published
1987-01-00
Pages
51-7
Language
English
Region
Netherlands
NLM ID
9106343
External Links
PubMed source DOI: 10.1007/BF00017986 View journal details
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