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PMID: 2426580 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Phosphatidylinositol kinase activity in virus-transformed and nontransformed cells.

Molecular and cellular biology ·Vol. 5 ·No. 9 ·1985-09-00 ·Pages 2399-404

Sugano S, Hanafusa H

Abstract

We assayed phosphatidylinositol (PI) kinase (EC 2.7.1.67) activity in detergent extracts of nontransformed or virus-transformed cells. Nontransformed chicken embryo fibroblasts (CEF) contain PI kinase activity with an apparent specific activity of 20 pmol/min per mg of protein. This activity sedimented as a single peak with a molecular weight of approximately 60,000 in a glycerol gradient, although immunoprecipitation with anti-p60src sera showed that the PI kinase activity is distinct from p60c-src. Extracts from CEF transformed by Rous sarcoma virus, Fujinami sarcoma virus, or avian sarcoma virus UR2 showed no elevation of PI kinase activity over nontransformed CEF. Removal of the oncogene products from extracts by immunoprecipitation did not change the level of PI kinase activity in extracts, suggesting that putative virus-coded PI kinases do not make a significant contribution to overall levels of PI kinase activity in transformed cells. Additionally, P140gag-fps was separated from cellular PI kinase by phosphocellulose chromatography. This partially purified fraction contained low PI kinase activity distinct from P140gag-fps, indicating that P140gag-fps has no detectable PI kinase activity.

MeSH Terms
1-Phosphatidylinositol 4-Kinase Animals Avian Sarcoma Viruses/enzymology,physiology Cell Transformation, Viral Chick Embryo Fibroblasts/enzymology Oncogene Protein pp60(v-src) Phosphotransferases/analysis,physiology Proto-Oncogene Proteins/physiology Proto-Oncogene Proteins pp60(c-src) Retroviridae Proteins/analysis,physiology
Chemicals
Proto-Oncogene Proteins Retroviridae Proteins Phosphotransferases 1-Phosphatidylinositol 4-Kinase Oncogene Protein pp60(v-src) Proto-Oncogene Proteins pp60(c-src)
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Sugano S
Hanafusa H
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37 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.5.9.2399-2404.1985
Published
1985-09-00
Pages
2399-404
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC366967
Subset
IM
Grants
NCI NIH HHS · CA14935 · United States
External Links
PubMed source DOI: 10.1128/mcb.5.9.2399-2404.1985 PMC Full Text View journal details
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