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PMID: 2425360 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Clustered arrangement of keratin intermediate filament genes.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 83 ·No. 14 ·1986-07-00 ·Pages 5048-52

Powell BC, Cam GR, Fietz MJ, Rogers GE

Abstract

We report here that component members of the keratin intermediate filament (IF) type I and type II gene families of sheep are closely linked but apparently the two families are not. Nine genes, accounting for up to half of the keratin IF gene repertoire, were mapped in four cosmid clones and the linkage between the genes ranged from several kilobases to 20 kilobases. In one cosmid, three tandem type I genes had the same transcriptional arrangement and were regularly spaced. In another cosmid, tandem genes encoding type II keratins were identified and, surprisingly, a solitary exon was discovered in the intergene region between the two type II genes. In a normal gene this exon encodes one of the most conserved amino acid regions of IF proteins, the C-terminal end of the alpha-helical core. Homologous C-terminal protein subdomains were encoded by two wool keratin type II genes and we suggest that this arrangement may also exist in the other wool keratin type II genes.

MeSH Terms
Animals Base Sequence Cosmids DNA, Recombinant/analysis Genes Keratins/genetics Sheep Skin/analysis Wool/analysis
Chemicals
DNA, Recombinant Keratins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Powell B C
Cam G R
Fietz M J
Rogers G E
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34 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.83.14.5048
Published
1986-07-00
Pages
5048-52
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC323887
Subset
IM
Databases
GENBANK
M13679
External Links
PubMed source DOI: 10.1073/pnas.83.14.5048 PMC Full Text View journal details
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