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PMID: 10872323 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Neurofilament architecture combines structural principles of intermediate filaments with carboxy-terminal extensions increasing in size between triplet proteins.

The EMBO journal ·Vol. 2 ·No. 8 ·1983-00-00 ·Pages 1295-302

Geisler N, Kaufmann E, Fischer S, Plessmann U, Weber K

Abstract

Mammalian neurofilament triplet proteins (68 K, 160 K and 200 K) have been correlated by a biochemical, immunological and protein chemical study. The 160 K and 200 K triplet proteins are intermediate filament proteins in their own right, since they reveal the alpha-helical coiled-coil rod domain analyzed in detail for the 68 K protein. Triplet proteins display two distinct arrays. Their amino-terminal region built analogously to non-neuronal intermediate filament proteins should allow a co-polymerization process via the interaction of coiled-coil domains. The extra mass of all triplet proteins is allocated to carboxy-terminally located extensions of increasing size and unique amino acid sequences. These may provide highly charged scaffolds suitable for interactions with other neuronal components. Such a domain of 68 K reveals, in sequence analysis, 47 glutamic acids within 106 residues. The epitope recognized by a monoclonal antibody reacting probably with all intermediate filament proteins has been mapped. It is located within the last 20 residues of the rods, where six distinct intermediate filament proteins point to a consensus sequence.

MeSH Terms
Amino Acid Sequence Animals Antibodies, Monoclonal/immunology Chymotrypsin Epitope Mapping Epitopes, B-Lymphocyte/immunology Intermediate Filaments/metabolism Molecular Sequence Data Neurofilament Proteins/chemistry,immunology,metabolism Protein Structure, Tertiary Swine
Chemicals
Antibodies, Monoclonal Epitopes, B-Lymphocyte Neurofilament Proteins Chymotrypsin
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Geisler N
Max-Planck-Institute for Biophysical Chemistry, Göttingen, FRG.
Kaufmann E
Fischer S
Plessmann U
Weber K
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1983-00-00
Pages
1295-302
Language
English
Region
England
NLM ID
8208664
PMCID
PMC555275
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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