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PMID: 2419837 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The trypanosome spliced leader small RNA gene family: stage-specific modification of one of several similar dispersed genes.

Nucleic acids research ·Vol. 14 ·No. 4 ·1986-02-25 ·Pages 1703-18

Parsons M, Nelson RG, Agabian N

Abstract

Diverse mRNAs of Trypanosoma brucei possess the same 5' terminal 35 nucleotides, termed the spliced leader (SL), which appears to be derived from a separate 135 nucleotide transcript. This small SL RNA is encoded within a 1.4 kb unit of DNA which is tandemly reiterated in the genome. In addition, there are at least 4 orphon elements containing SL sequences dispersed from the tandem array. Here we show that during the trypanosome life cycle one of the SL orphons undergoes a stage-specific modification that prevents cleavage of an EcoRV site and we further demonstrate that although only one orphon is modified, three of the SL orphons are flanked by very similar sequences. Each of these contains SL reiteration units including the non-transcribed spacer DNA, suggesting that they did not originate through an RNA intermediate. In addition no evidence of direct repeats at the junction of 1.4 kb and non-1.4 kb DNA was observed. Finally, a phylogenetic survey indicates that while many trypanosomatid species possess similarly organized SL-like sequences, only the SL orphons of closely related subspecies of the T. brucei - T. evansi complex share similar flanking regions.

MeSH Terms
Animals Cloning, Molecular Gene Expression Regulation Genes Genetic Linkage Polymorphism, Genetic RNA/genetics RNA Splicing Repetitive Sequences, Nucleic Acid Species Specificity Trypanosoma brucei brucei/genetics
Chemicals
RNA
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Parsons M
Nelson R G
Agabian N
References (37)
37 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/14.4.1703
Published
1986-02-25
Pages
1703-18
Language
English
Region
England
NLM ID
0411011
PMCID
PMC339564
Subset
IM
Grants
NIAID NIH HHS · AI17309 · United States
NIAID NIH HHS · AI21975 · United States
Databases
GENBANK
X03581, X03582
External Links
PubMed source DOI: 10.1093/nar/14.4.1703 PMC Full Text View journal details
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