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PMID: 2417229 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Intracellular calcium regulates basolateral potassium channels in a chloride-secreting epithelium.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 82 ·No. 24 ·1985-12-00 ·Pages 8823-6

Welsh MJ, McCann JD

Abstract

The two individual cell membranes of epithelia are functionally coupled, so that changes in apical membrane conductance are paralleled by changes in basolateral K+ conductance. However, the signal that regulates basolateral K+ conductance, thereby coupling the two membranes, is unknown. We tested the hypothesis that the cellular calcium concentration, [Ca2+]c, may regulate basolateral K+ conductance in canine tracheal epithelium, a Cl- -secreting epithelium that shows marked membrane coupling. Three findings support the hypothesis. First, the intracellular Ca2+ antagonist 8-(diethylamino)octyl 3,4,5-trimethoxybenzoate hydrochloride (TMB-8) attenuated the secretory response. Second, the secretagogue epinephrine increased [Ca2+]c, as measured with quin-2. Third, we found a K+ channel that was activated by Ca2+ on the cytosolic side of the membrane. Thus, cytosolic Ca2+ regulates the basolateral K+ conductance and may be the signal responsible for functional coupling of the two cell membranes.

MeSH Terms
Animals Calcium/physiology Chlorides/metabolism Dogs Electric Conductivity Epinephrine/pharmacology Epithelium/physiology Gallic Acid/analogs & derivatives,pharmacology In Vitro Techniques Ion Channels/physiology Potassium/physiology Secretory Rate/drug effects Trachea/physiology
Chemicals
Chlorides Ion Channels 8-(N,N-diethylamino)octyl-3,4,5-trimethoxybenzoate Gallic Acid Potassium Calcium Epinephrine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Welsh M J
McCann J D
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31 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.82.24.8823
Published
1985-12-00
Pages
8823-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC391530
Subset
IM
Grants
NHLBI NIH HHS · HL-14388 · United States
NHLBI NIH HHS · HL-29851 · United States
External Links
PubMed source DOI: 10.1073/pnas.82.24.8823 PMC Full Text View journal details
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