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PMID: 2394688 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A biovar-specific signal of Rhizobium leguminosarum bv. viciae induces increased nodulation gene-inducing activity in root exudate of Vicia sativa subsp. nigra.

Journal of bacteriology ·Vol. 172 ·No. 9 ·1990-09-00 ·Pages 5394-401

van Brussel AA, Recourt K, Pees E, Spaink HP, Tak T, Wijffelman CA, Kijne JW, Lugtenberg BJ

Abstract

Flavonoids in root exudate of leguminous plants activate the transcription of Rhizobium genes involved in the formation of root nodules (nod genes). We report that inoculation with the homologous symbiont R. leguminosarum bv. viciae results in an increased nod gene-inducing activity (Ini) in root exudate of V. sativa subsp. nigra, whereas inoculation with heterologous Rhizobium strains results in exudates with nod gene-inducing activity comparable to that of uninfected plants. Ini can be demonstrated by using either of the isogenic indicator strains containing an inducible nod promoter fused to the Escherichia coli lacZ reporter gene and the regulatory nodD gene of R. leguminosarum bv. viciae, R. leguminosarum bv. trifolii, or R. meliloti. The presence of genes nodDABCEL of R. leguminosarum bv. viciae appeared to be essential for induction of Ini. Mutation of the genes nodI and nodJ causes a delay of Ini, whereas gene nodF appears to be required for both the timely appearance and the maximum level of Ini activity. The nodE gene is responsible for the biovar specificity of induction of Ini by Rhizobium spp. Ini is caused by a soluble heat-stable factor of rhizobial origin. This Rhizobium-produced Ini factor has an apparent molecular weight between 1,000 and 10,000 and does not originate from flavonoid precursors.

MeSH Terms
Cells, Cultured Gene Expression Regulation, Bacterial Genes, Bacterial Mutation Phenotype Plants/microbiology Plasmids Rhizobium/genetics Species Specificity Transcription, Genetic
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
van Brussel A A
Department of Plant Molecular Biology, Leiden University, Leiden, The Netherlands.
Recourt K
Pees E
Spaink H P
Tak T
Wijffelman C A
Kijne J W
Lugtenberg B J
References (25)
25 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.172.9.5394-5401.1990
Published
1990-09-00
Pages
5394-401
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC213204
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.172.9.5394-5401.1990 PMC Full Text View journal details
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