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PMID: 2388831 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A novel transcriptional activator (PO-B) binds between the TATA box and cap site of the pro-opiomelanocortin gene.

Nucleic acids research ·Vol. 18 ·No. 15 ·1990-08-11 ·Pages 4513-21

Riegel AT, Remenick J, Wolford RG, Berard DS, Hager GL

Abstract

We demonstrate that a factor (PO-B), detected in a number of mammalian cell lines, binds specifically between the TATA box and the transcription initiation site of the pituitary-specific pro-opiomelanocortin (POMC) gene. Methylation interference and DNAse 1 footprint analysis revealed that the binding site of this protein, -3 to -15, does not overlap the POMC TATA box or cap site. Mutation of the PO-B binding site significantly decreased the transcriptional activity of the POMC promoter after transient transfection into the ATt-20 mouse pituitary tumor cell line and also in in vitro transcription assays. In contrast, mutation of the TATA box produced no overall decrease in transcription in vivo but induced multiple heterogeneous start sites in vitro. A vector harboring the PO-B site alone was unable to direct transcription initiation. PO-B represents a new transcription factor that may be able to facilitate POMC gene expression by interacting with components of the transcription initiation complex.

MeSH Terms
Animals Base Sequence Binding Sites Cell Line Deoxyribonuclease I/metabolism Gene Expression Regulation HeLa Cells Humans Methylation Mice Molecular Sequence Data Mutation Pituitary Gland/cytology,metabolism Pro-Opiomelanocortin/genetics Promoter Regions, Genetic Transcription Factors/genetics,metabolism Transcription, Genetic Transfection
Chemicals
Transcription Factors Pro-Opiomelanocortin Deoxyribonuclease I
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Riegel A T
Hormone Action and Oncogenesis Section, National Cancer Institute, Bethesda, MD 20892.
Remenick J
Wolford R G
Berard D S
Hager G L
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/18.15.4513
Published
1990-08-11
Pages
4513-21
Language
English
Region
England
NLM ID
0411011
PMCID
PMC331272
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/18.15.4513 PMC Full Text View journal details
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