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PMID: 2550796 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Independent glucocorticoid induction and repression of two contiguous responsive genes.

Molecular and cellular biology ·Vol. 9 ·No. 7 ·1989-07-00 ·Pages 3127-31

Charron J, Richard-Foy H, Berard DS, Hager GL, Drouin J

Abstract

Specific DNA sequence elements which contain binding sites for the glucocorticoid receptor mediate the action of glucocorticoid hormones on gene transcription. In glucocorticoid-inducible genes, these glucocorticoid-responsive elements behave as hormone-inducible enhancers of transcription. We have taken advantage of the bovine papillomavirus (BPV) system to test the stringency of glucocorticoid regulation of transcription. BPV episomes were constructed to contain two hormone-regulated transcription units in close proximity; one transcription unit is under control of a glucocorticoid-inducible promoter (mouse mammary tumor virus) while the other is under control of a glucocorticoid-inhibited promoter (pro-opiomelanocortin). Glucocorticoids independently regulated transcription of the two physically linked transcription units, irrespective of their relative orientation and of their proximity on the BPV episomes. This result contrasts with the so-called position-independent activity of enhancers and suggests that the multicomponent organization of eucaryotic promoters restricts the action of hormone-responsive regulatory elements to a specific transcription unit, thus accounting for the stringency of hormonal regulation observed in vivo.

MeSH Terms
Animals Cell Line, Transformed DNA/genetics Glucocorticoids/physiology Immunoblotting Mammary Tumor Virus, Mouse/genetics Papillomaviridae/genetics Plasmids Pro-Opiomelanocortin/genetics Promoter Regions, Genetic Rats
Chemicals
Glucocorticoids Pro-Opiomelanocortin DNA
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Charron J
Laboratoire de Génétique Moléculaire, Institut de Recherches Cliniques de Montréal, Quebec, Canada.
Richard-Foy H
Berard D S
Hager G L
Drouin J
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.9.7.3127-3131.1989
Published
1989-07-00
Pages
3127-31
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC362787
Subset
IM
External Links
PubMed source DOI: 10.1128/mcb.9.7.3127-3131.1989 PMC Full Text View journal details
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