Home LiteratureArticle Details
PMID: 2377616 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Sequence analysis and protein import studies of an outer chloroplast envelope polypeptide.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 87 ·No. 15 ·1990-08-00 ·Pages 5778-82

Salomon M, Fischer K, Flügge UI, Soll J

Abstract

A chloroplast outer envelope membrane protein was cloned and sequenced and from the sequence it was possible to deduce a polypeptide of 6.7 kDa. It has only one membrane-spanning region; the C terminus extends into the cytosol, whereas the N terminus is exposed to the space between the two envelope membranes. The protein was synthesized in an in vitro transcription-translation system to study its routing into isolated chloroplasts. The import studies revealed that the 6.7-kDa protein followed a different and heretofore undescribed translocation pathway in the respect that (i) it does not have a cleavable transit sequence, (ii) it does not require ATP hydrolysis for import, and (iii) protease-sensitive components that are responsible for recognition of precursor proteins destined for the inside of the chloroplasts are not involved in routing the 6.7-kDa polypeptide to the outer chloroplast envelope.

MeSH Terms
Amino Acid Sequence Base Sequence Chloroplasts/metabolism Intracellular Membranes/metabolism Kinetics Membrane Proteins/genetics,metabolism Molecular Sequence Data Plants/genetics,metabolism Protein Biosynthesis Protein Conformation Protein Processing, Post-Translational Thermodynamics
Chemicals
Membrane Proteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Salomon M
Botanisches Institut, Universität Munich, Federal Republic of Germany.
Fischer K
Flügge U I
Soll J
References (28)
28 references, click to expand
  1. Thermolysin is a suitable protease for probing the surface of intact pea chloroplasts.
    Plant Physiol. 1984 Jul;75(3):675-8 PMID: 16663686
  2. ATP is required for the binding of precursor proteins to chloroplasts.
    J Biol Chem. 1989 Apr 25;264(12):6724-9 PMID: 2708339
  3. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.
    J Mol Biol. 1977 Jun 15;113(1):237-51 PMID: 881736
  4. Role of cytochrome c heme lyase in the import of cytochrome c into mitochondria.
    J Biol Chem. 1988 Dec 15;263(35):19034-42 PMID: 2848813
  5. COPPER ENZYMES IN ISOLATED CHLOROPLASTS. POLYPHENOLOXIDASE IN BETA VULGARIS.
    Plant Physiol. 1949 Jan;24(1):1-15 PMID: 16654194
  6. A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels.
    Eur J Biochem. 1974 Jul 1;46(1):83-8 PMID: 4850204
  7. Transport of proteins into chloroplasts. Binding of nuclear-coded chloroplast proteins to the chloroplast envelope.
    Eur J Biochem. 1982 Aug;126(1):143-8 PMID: 6181990
  8. The triose phosphate-3-phosphoglycerate-phosphate translocator from spinach chloroplasts: nucleotide sequence of a full-length cDNA clone and import of the in vitro synthesized precursor protein into chloroplasts.
    EMBO J. 1989 Jan;8(1):39-46 PMID: 2714257
  9. Separation and characterization of inner and outer envelope membranes of pea chloroplasts.
    Proc Natl Acad Sci U S A. 1981 Jun;78(6):3595-9 PMID: 16593034
  10. A simple method for displaying the hydropathic character of a protein.
    J Mol Biol. 1982 May 5;157(1):105-32 PMID: 7108955
  11. Preferential lipid association and mode of penetration of apocytochrome c in mixed model membranes as monitored by tryptophanyl fluorescence quenching using brominated phospholipids.
    Biochim Biophys Acta. 1987 Feb 12;897(1):1-4 PMID: 3026475
  12. Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa.
    Anal Biochem. 1987 Nov 1;166(2):368-79 PMID: 2449095
  13. The nuclear-coded chloroplast 22-kDa heat-shock protein of Chlamydomonas. Evidence for translocation into the organelle without a processing step.
    Eur J Biochem. 1989 Jul 1;182(3):539-46 PMID: 2473899
  14. Import pathways of precursor proteins into mitochondria: multiple receptor sites are followed by a common membrane insertion site.
    J Cell Biol. 1988 Dec;107(6 Pt 2):2483-90 PMID: 2974457
  15. Precursors to two nuclear-encoded chloroplast proteins bind to the outer envelope membrane before being imported into chloroplasts.
    J Biol Chem. 1985 Mar 25;260(6):3691-6 PMID: 3882712
  16. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  17. Transport of proteins into chloroplasts. Partial purification of a chloroplast protease involved in the processing of important precursor polypeptides.
    Eur J Biochem. 1984 Jul 16;142(2):337-42 PMID: 6430703
  18. Different transport pathways of individual precursor proteins in mitochondria.
    Eur J Biochem. 1981 Jun 1;116(3):455-60 PMID: 6167439
  19. Localization of polypeptides to the cytosolic side of the outer envelope membrane of spinach chloroplasts.
    J Biol Chem. 1983 Aug 25;258(16):10000-6 PMID: 6350279
  20. Energy dependence of protein translocation into chloroplasts.
    Eur J Biochem. 1986 Nov 3;160(3):563-70 PMID: 3780721
  21. Slow Passive Diffusion of Orthophosphate between Intact Isolated Chloroplasts and Suspending Medium.
    Plant Physiol. 1981 Mar;67(3):470-3 PMID: 16661696
  22. Translocation of proteins across the mitochondrial inner membrane, but not into the outer membrane, requires nucleoside triphosphates in the matrix.
    Proc Natl Acad Sci U S A. 1989 Nov;86(21):8432-6 PMID: 2554316
  23. Yeast RNA polymerase II genes: isolation with antibody probes.
    Science. 1983 Nov 18;222(4625):778-82 PMID: 6356359
  24. Mitochondrial protein import.
    Biochim Biophys Acta. 1989 Jan 18;988(1):1-45 PMID: 2642391
  25. Preparation and characterization of membrane fractions enriched in outer and inner envelope membranes from spinach chloroplasts. I. Electrophoretic and immunochemical analyses.
    J Biol Chem. 1983 Nov 10;258(21):13273-80 PMID: 6415062
  26. The plastid envelope membranes: their structure, composition, and role in chloroplast biogenesis.
    Subcell Biochem. 1984;10:1-84 PMID: 6382702
  27. Efficient in vitro import of a cytosolic heat shock protein into pea chloroplasts.
    Proc Natl Acad Sci U S A. 1986 Aug;83(15):5502-6 PMID: 16593735
  28. Characterization of envelope membrane polypeptides from spinach chloroplasts.
    J Biol Chem. 1982 Jan 25;257(2):1095-101 PMID: 6798029
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.87.15.5778
Published
1990-08-00
Pages
5778-82
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC54411
Subset
IM
Databases
GENBANK
M35665, M36587
External Links
PubMed source DOI: 10.1073/pnas.87.15.5778 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2