Separation and characterization of inner and outer envelope membranes of pea chloroplasts.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 78 ·No. 6 ·1981-06-00 ·Pages 3595-9

Cline K, Andrews J, Mersey B, Newcomb EH, Keegstra K

Abstract

A procedure for purifying the chloroplast envelope subfractionates it into two membrane fractions of comparable quantities. This procedure differs from previous ones in that the chloroplasts are ruptured by freezing and thawing in hypertonic medium rather than by osmotic shock. The two membrane fractions have qualitatively similar polar lipid compositions but differ in their content of individual lipids, specifically monogalactosyldiacylglycerol and phosphatidylcholine. The two fractions also differ in their constituent polypeptides and in their appearance when examined by electron microscopy. The light (density = 1.08 g/ml) and heavy (density = 1.13 g/ml) membrane fractions have been tentatively identified as the outer and inner envelope membranes, respectively.

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Cline K

Department of Botany, University of Wisconsin, Madison, Wisconsin 53706.

Andrews J

Mersey B

Newcomb E H

Keegstra K

References (10)

10 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.78.6.3595

Published

1981-06-00

Pages

3595-9

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC319617

External Links

PubMed source DOI: 10.1073/pnas.78.6.3595 PMC Full Text View journal details

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Separation and characterization of inner and outer envelope membranes of pea chloroplasts.

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