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PMID: 23023225 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Integrins β1 and β3 exhibit distinct dynamic nanoscale organizations inside focal adhesions.

Nature cell biology ·Vol. 14 ·No. 10 ·2012-10-00 ·Pages 1057-67

Rossier O, Octeau V, Sibarita JB, Leduc C, Tessier B, Nair D, Gatterdam V, Destaing O, Albigès-Rizo C, Tampé R, Cognet L, Choquet D, Lounis B, Giannone G

Abstract

Integrins in focal adhesions (FAs) mediate adhesion and force transmission to extracellular matrices essential for cell motility, proliferation and differentiation. Different fibronectin-binding integrins, simultaneously present in FAs, perform distinct functions. Yet, how integrin dynamics control biochemical and biomechanical processes in FAs is still elusive. Using single-protein tracking and super-resolution imaging we revealed the dynamic nano-organizations of integrins and talin inside FAs. Integrins reside in FAs through free-diffusion and immobilization cycles. Integrin activation promotes immobilization, stabilized in FAs by simultaneous connection to fibronectin and actin-binding proteins. Talin is recruited in FAs directly from the cytosol without membrane free-diffusion, restricting integrin immobilization to FAs. Immobilized β3-integrins are enriched and stationary within FAs, whereas immobilized β1-integrins are less enriched and exhibit rearward movements. Talin is enriched and mainly stationary, but also exhibited rearward movements in FAs, consistent with stable connections with both β-integrins. Thus, differential transmission of actin motion to fibronectin occurs through specific integrins within FAs.

MeSH Terms
Animals Fibronectins/metabolism Focal Adhesions/metabolism Integrin beta1/metabolism Integrin beta3/metabolism Mice Microfilament Proteins/metabolism Protein Binding Talin/metabolism
Chemicals
Fibronectins Integrin beta1 Integrin beta3 Microfilament Proteins Talin talin protein, mouse
Authors & Affiliations
14 authors, click to expand affiliations / ORCID
Rossier Olivier
Interdisciplinary Institute for Neuroscience, University of Bordeaux, UMR 5297, F-33000 Bordeaux, France.
Octeau Vivien
Sibarita Jean-Baptiste
Leduc Cécile
Tessier Béatrice
Nair Deepak
Gatterdam Volker
Destaing Olivier
Albigès-Rizo Corinne
Tampé Robert
Cognet Laurent
Choquet Daniel
Lounis Brahim
Giannone Grégory
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Article Info
Journal
Nature cell biology
Abbr.
Nat Cell Biol
ISSN
1476-4679
DOI
10.1038/ncb2588
Published
2012-10-00
Epub
2012-00-30
Pages
1057-67
Language
English
Region
England
NLM ID
100890575
Subset
IM
Grants
European Research Council · 232942 · International
Corrections
ErratumIn
-
External Links
PubMed source DOI: 10.1038/ncb2588 View journal details
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