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PMID: 2300557 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Transcription and cap trimethylation of a nematode spliced leader RNA in a cell-free system.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 87 ·No. 2 ·1990-01-00 ·Pages 709-13

Maroney PA, Hannon GJ, Nilsen TW

Abstract

Maturation of a fraction of mRNAs in nematodes involves the acquisition of a common 5' terminal spliced leader sequence derived from a nonpolyadenylylated spliced leader RNA by trans splicing. We have developed a cell-free system prepared from Ascaris lumbricoides embryos that accurately and efficiently synthesized the spliced leader RNA of A. lumbricoides. Transcription of the spliced leader RNA was catalyzed by RNA polymerase II, and the majority of the spliced leader RNAs synthesized in vitro possessed a trimethylguanosine cap structure identical to that found on in vivo-synthesized spliced leader RNA.

MeSH Terms
Animals Ascaris/embryology,genetics Base Sequence Cell-Free System Embryo, Nonmammalian/metabolism Methylation Molecular Sequence Data Nucleotide Mapping RNA Caps/genetics,metabolism RNA Polymerase II/metabolism RNA Splicing RNA, Messenger/genetics RNA, Small Nuclear/genetics Transcription, Genetic
Chemicals
RNA Caps RNA, Messenger RNA, Small Nuclear RNA Polymerase II
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Maroney P A
Department of Molecular Biology and Microbiology, Case Western Reserve University, Cleveland, OH 44106.
Hannon G J
Nilsen T W
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19 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.87.2.709
Published
1990-01-00
Pages
709-13
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC53335
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.87.2.709 PMC Full Text View journal details
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