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PMID: 3186698 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

A 22-nucleotide spliced leader sequence in the human parasitic nematode Brugia malayi is identical to the trans-spliced leader exon in Caenorhabditis elegans.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 85 ·No. 21 ·1988-11-00 ·Pages 7932-6

Takacs AM, Denker JA, Perrine KG, Maroney PA, Nilsen TW

Abstract

The mRNAs encoding a 63-kDa antigen in the human parasitic nematode Brugia Malayi contain a spliced leader sequence of 22 nucleotides (nt) that is identical to the trans-spliced leader found on certain actin mRNAs in the distantly related nematode Caenorhabditis elegans. The 22-nt sequence does not appear to be encoded near the 63-kDa genes but is present in multiple copies in several locations within the parasite genome, including the 5S rRNA gene repeat. The 5S-linked copies of the 22-nt sequence are transcribed to yield a 109-nt nonpolyadenylated RNA with the 22-nt leader sequence at its 5' end. We suggest that the 22-nt leader is acquired by 63-kDa antigen mRNAs through trans-splicing. These results indicate that trans-splicing is widespread in nematodes and argue for the functional significance of the 22-nt spliced leader exon in nematode mRNA metabolism.

MeSH Terms
Animals Base Sequence Brugia/genetics Caenorhabditis/genetics Humans Molecular Sequence Data Molecular Weight RNA Splicing RNA, Ribosomal, 5S/analysis Repetitive Sequences, Nucleic Acid Transcription, Genetic
Chemicals
RNA, Ribosomal, 5S
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Takacs A M
Department of Molecular Biology and Microbiology, Case Western Reserve University, School of Medicine, Cleveland, OH 44106.
Denker J A
Perrine K G
Maroney P A
Nilsen T W
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.85.21.7932
Published
1988-11-00
Pages
7932-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC282327
Subset
IM
Databases
GENBANK
M23324
External Links
PubMed source DOI: 10.1073/pnas.85.21.7932 PMC Full Text View journal details
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