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PMID: 2747746 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Accurate and efficient RNA polymerase III transcription in a cell-free extract prepared from Ascaris suum embryos.

Molecular and biochemical parasitology ·Vol. 35 ·No. 3 ·1989-07-00 ·Pages 277-83

Maroney PA, Hannon GJ, Nilsen TW

Abstract

High-speed supernatant (S100) extracts derived from homogenized Ascaris suum embryos efficiently transcribe added RNA polymerase III templates including cloned 5S rRNA genes of the filarial parasite Brugia malayi. Several criteria, including two-dimensional RNase T1 oligonucleotide fingerprint analysis, indicate that in vitro transcription is accurately initiated and terminated.

MeSH Terms

Amanitins/pharmacology Animals Ascaris/genetics Brugia/genetics Cell-Free System Cloning, Molecular DNA-Directed RNA Polymerases/genetics Female Nucleic Acid Hybridization RNA Polymerase III/genetics RNA, Ribosomal, 5S/biosynthesis Terminator Regions, Genetic Transcription, Genetic Xenopus/genetics

Chemicals

Amanitins RNA, Ribosomal, 5S DNA-Directed RNA Polymerases RNA Polymerase III

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Maroney P A

Department of Molecular Biology and Microbiology, Case Western Reserve University School of Medicine, Cleveland, OH.

Hannon G J

Nilsen T W

Article Info

Journal

Molecular and biochemical parasitology

Abbr.

Mol Biochem Parasitol

ISSN

0166-6851

DOI

10.1016/0166-6851(89)90214-4

Published

1989-07-00

Pages

277-83

Language

English

Region

Netherlands

NLM ID

8006324

Subset

Grants

NIGMS NIH HHS · GM08056 · United States

External Links

PubMed source DOI: 10.1016/0166-6851(89)90214-4 View journal details

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Accurate and efficient RNA polymerase III transcription in a cell-free extract prepared from Ascaris suum embryos.

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