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PMID: 22749399 Published · ppublish English Journal Article Research Support, N.I.H., Extramural

A bridging model for persistence of a polycomb group protein complex through DNA replication in vitro.

Molecular cell ·Vol. 46 ·No. 6 ·2012-06-29 ·Pages 784-96

Lo SM, Follmer NE, Lengsfeld BM, Madamba EV, Seong S, Grau DJ, Francis NJ

Abstract

Epigenetic regulation may involve heritable chromatin states, but how chromatin features can be inherited through DNA replication is incompletely understood. We address this question using cell-free replication of chromatin. Previously, we showed that a Polycomb group complex, PRC1, remains continuously associated with chromatin through DNA replication. Here we investigate the mechanism of persistence. We find that a single PRC1 subunit, Posterior sex combs (PSC), can reconstitute persistence through DNA replication. PSC binds nucleosomes and self-interacts, bridging nucleosomes into a stable, oligomeric structure. Within these structures, individual PSC-chromatin contacts are dynamic. Stable association of PSC with chromatin, including through DNA replication, depends on PSC-PSC interactions. Our data suggest that labile individual PSC-chromatin contacts allow passage of the DNA replication machinery while PSC-PSC interactions prevent PSC from dissociating, allowing it to rebind to replicated chromatin. This mechanism may allow inheritance of chromatin proteins including PRC1 through DNA replication to maintain chromatin states.

MeSH Terms
Animals Chromatin/metabolism Chromatin Assembly and Disassembly DNA/metabolism DNA Replication Drosophila/metabolism Drosophila Proteins/metabolism Humans Nucleosomes/metabolism Polycomb-Group Proteins Repressor Proteins/chemistry,metabolism
Chemicals
Chromatin Drosophila Proteins Nucleosomes Polycomb-Group Proteins Repressor Proteins DNA
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Lo Stanley M
Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA.
Follmer Nicole E
Lengsfeld Bettina M
Madamba Egbert V
Seong Samuel
Grau Daniel J
Francis Nicole J
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Article Info
Journal
Molecular cell
Abbr.
Mol Cell
ISSN
1097-4164
DOI
10.1016/j.molcel.2012.05.038
Published
2012-06-29
Pages
784-96
Language
English
Region
United States
NLM ID
9802571
PMCID
PMC3389374
Subset
IM
Grants
NIGMS NIH HHS · R01 GM078456 · United States
NIGMS NIH HHS · GM78456 · United States
External Links
PubMed source DOI: 10.1016/j.molcel.2012.05.038 PMC Full Text View journal details
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