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PMID: 2274040 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Sequence, organization and expression of the core histone genes of Aspergillus nidulans.

Molecular & general genetics : MGG ·Vol. 222 ·No. 2-3 ·1990-07-00 ·Pages 416-24

Ehinger A, Denison SH, May GS

Abstract

The core histone gene family of Aspergillus nidulans was characterized. The H2A, H2B and H3 genes are unique in the A. nidulans genome. In contrast there are two H4 genes, H4.1 and H4.2. As previously reported for the H2A gene (May and Morris 1987) introns also interrupt the other core histone genes. The H2B gene, like the H2A gene, is interrupted by three introns, the H3 and H4.1 gene are each interrupted by two introns and the H4.2 gene contains one intron. The position of the single intron in H4.2 is the same as that the first intron of the H4.1 gene. The H2A and H2B genes are arranged as a gene pair separated by approximately 600 bp and are divergently transcribed. The H3 and H4.1 genes are similarly arranged and are separated by approximately 800 bp. The H4.2 gene is not closely linked to either the H2A-H2B or H3-H4.1 gene pairs. Using pulse field gel electrophoresis an electrophoretic karyotype was established for A. nidulans. This karyotype was used to assign the H3-H4.1 gene pair and the H4.2 gene to linkage group VIII and the H2A-H2B gene pair to either linkage group III or VI. The abundance of each of the histone messenger RNAs was determined to be cell cycle regulated but the abundance of the H4.2 mRNA appears to be regulated differently from the others.

MeSH Terms
Amino Acid Sequence Aspergillus nidulans/genetics Base Sequence Blotting, Southern DNA, Fungal/genetics,isolation & purification Fungal Proteins/biosynthesis,genetics Gene Expression Regulation, Fungal Genes, Fungal Genetic Linkage Histones/biosynthesis,genetics Introns Karyotyping Molecular Sequence Data Multigene Family Restriction Mapping Sequence Homology, Nucleic Acid Transcription, Genetic
Chemicals
DNA, Fungal Fungal Proteins Histones
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Ehinger A
Department of Cell Biology, Baylor College of Medicine, Houston, TX 77030.
Denison S H
May G S
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00633848
Published
1990-07-00
Pages
416-24
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
Grants
NIGMS NIH HHS · GM41626 · United States
NCRR NIH HHS · RR-05425 · United States
External Links
PubMed source DOI: 10.1007/BF00633848
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