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PMID: 2270076 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Possible involvement of the ugpA gene product in the stable maintenance of mini-F plasmid in Escherichia coli.

Molecular & general genetics : MGG ·Vol. 223 ·No. 3 ·1990-09-00 ·Pages 361-8

Ezaki B, Mori H, Ogura T, Hiraga S

Abstract

The seg-3 mutant Escherichia coli does not support the maintenance of mini-F plasmid at 42 degrees C. We cloned the chromosomal DNA segment of the wild-type strain W3110 that complements the Seg- phenotype of this mutant. Cleavage mapping of this segment showed that it was derived from the 76-min region of the E. coli chromosome map. Complementation tests using plasmids carrying subcloned DNA segments suggested that the seg-3 mutant carried two mutations that additively affected the maintenance of mini-F plasmid; one was in the ugpA gene and the other was presumably in the rpoH gene. We generated a disrupted ugpA null mutant and found that the mini-F plasmid was unstable in this ugpA null mutant even at 30 degrees C. This suggests that the ugpA gene product is required for the stable maintenance of mini-F plasmid.

Related Genes
ugpA
MeSH Terms
Bacterial Proteins/genetics,metabolism Biological Transport Cloning, Molecular Escherichia coli/genetics F Factor Genes, Bacterial Genetic Complementation Test Heat-Shock Proteins/metabolism Mutation Phenotype Phosphates/metabolism Restriction Mapping
Chemicals
Bacterial Proteins Heat-Shock Proteins Phosphates
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Ezaki B
Department of Molecular Genetics, Kumamoto University Medical School, Japan.
Mori H
Ogura T
Hiraga S
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35 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00264441
Published
1990-09-00
Pages
361-8
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00264441
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