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PMID: 2183003 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Maintenance of plasmids in HU and IHF mutants of Escherichia coli.

Molecular & general genetics : MGG ·Vol. 220 ·No. 2 ·1990-01-00 ·Pages 197-203

Ogura T, Niki H, Kano Y, Imamoto F, Hiraga S

Abstract

Complementation and sequencing analyses revealed that the hopD mutants, which could not support stable maintenance of mini-F plasmids (Niki et al. 1988), had mutations in the hupB gene, and that the hopD410 mutation was an ochre mutation at the 5th Gln position of HU-1. Maintenance and stability of various plasmids, mini-P1 plasmids, mini-F plasmids, and oriC plasmids, were studied in the hupA and hupB mutants (HU mutants), and himA and hip mutants (IHF mutants). Mini-P1 plasmids and mini-F plasmids could not be introduced into the delta hupA-delta hupB double deletion mutant. Replication of mini-F plasmids was partially inhibited in the hupB mutants, including the delta hupB and hopD(hupB) mutants, whereas replication of oriC plasmids was not significantly affected even in the delta hupA-delta hupB double deletion mutant. The mini-P1 plasmid was slightly unstable in the himA-hip mutant, whereas the mini-F plasmid was stable.

MeSH Terms
Amino Acid Sequence Bacterial Proteins/genetics Bacteriocin Plasmids Base Sequence DNA-Binding Proteins/genetics Escherichia coli/genetics F Factor Genes, Bacterial Genetic Complementation Test Integration Host Factors Molecular Sequence Data Mutation Plasmids Restriction Mapping Time Factors Transformation, Bacterial
Chemicals
Bacterial Proteins DNA-Binding Proteins Integration Host Factors histone-like protein HU, bacteria
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Ogura T
Department of Molecular Genetics, Kumamoto University Medical School, Japan.
Niki H
Kano Y
Imamoto F
Hiraga S
References (44)
44 references, click to expand
  1. Requirement of the Escherichia coli dnaA gene function for ori-2-dependent mini-F plasmid replication.
    J Bacteriol. 1987 Apr;169(4):1724-30 PMID: 3031019
  2. Lambda replacement vectors carrying polylinker sequences.
    J Mol Biol. 1983 Nov 15;170(4):827-42 PMID: 6315951
  3. Duplex opening by dnaA protein at novel sequences in initiation of replication at the origin of the E. coli chromosome.
    Cell. 1988 Mar 11;52(5):743-55 PMID: 2830993
  4. Partition mechanism of F plasmid: two plasmid gene-encoded products and a cis-acting region are involved in partition.
    Cell. 1983 Feb;32(2):351-60 PMID: 6297791
  5. Participation of hup gene product in replicative transposition of Mu phage in Escherichia coli.
    Gene. 1989;76(2):353-8 PMID: 2666261
  6. Sequence of the Escherichia coli pheST operon and identification of the himA gene.
    J Bacteriol. 1985 Aug;163(2):787-91 PMID: 2991205
  7. Genetic characterization of the gene hupA encoding the HU-2 protein of Escherichia coli.
    Gene. 1988 Sep 30;69(2):331-5 PMID: 3069589
  8. Construction and characterization of the deletion mutant of hupA and hupB genes in Escherichia coli.
    J Mol Biol. 1988 Dec 5;204(3):581-91 PMID: 3066907
  9. Inhibition of initiation of mini-F plasmid replication in temperature-sensitive mafA mutants of Escherichia coli K-12.
    Plasmid. 1982 Nov;8(3):287-98 PMID: 6757995
  10. Participation of hup gene product in ori2-dependent replication of fertility plasmid F.
    Gene. 1988 Oct 30;70(2):393-7 PMID: 3063607
  11. Structure and function of the F plasmid genes essential for partitioning.
    J Mol Biol. 1986 Nov 5;192(1):1-15 PMID: 3029390
  12. A mutant of Escherichia coli incapable of supporting vegetative replication of F-like plasmids.
    J Mol Biol. 1976 Nov;108(1):25-41 PMID: 794490
  13. Primary structure of the hip gene of Escherichia coli and of its product, the beta subunit of integration host factor.
    J Mol Biol. 1985 May 25;183(2):117-28 PMID: 3159903
  14. Participation of Escherichia coli integration host factor in the P1 plasmid partition system.
    Proc Natl Acad Sci U S A. 1988 Sep;85(18):6657-61 PMID: 2842786
  15. Replication of pSC101: effects of mutations in the E. coli DNA binding protein IHF.
    Mol Gen Genet. 1986 Jul;204(1):85-9 PMID: 3528758
  16. Host control of plasmid replication: requirement for the sigma factor sigma 32 in transcription of mini-F replication initiator gene.
    Proc Natl Acad Sci U S A. 1987 Dec;84(24):8849-53 PMID: 2447584
  17. Transcriptional activation of initiation of replication from the E. coli chromosomal origin: an RNA-DNA hybrid near oriC.
    Cell. 1988 Oct 7;55(1):113-23 PMID: 2458841
  18. Host protein requirements for in vitro site-specific DNA inversion.
    Cell. 1986 Aug 15;46(4):531-9 PMID: 3524854
  19. Primary structures of two homologous ribosome-associated DNA-binding proteins of Escherichia coli.
    FEBS Lett. 1978 Dec 15;96(2):395-8 PMID: 215461
  20. New M13 vectors for cloning.
    Methods Enzymol. 1983;101:20-78 PMID: 6310323
  21. A defined system for the DNA strand-transfer reaction at the initiation of bacteriophage Mu transposition: protein and DNA substrate requirements.
    Proc Natl Acad Sci U S A. 1985 Nov;82(22):7570-4 PMID: 2999771
  22. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  23. Genetic characterization of the gene hupB encoding the HU-1 protein of Escherichia coli.
    Gene. 1986;45(1):37-44 PMID: 3536664
  24. Localization of the HU protein on the Escherichia coli nucleoid.
    Cold Spring Harb Symp Quant Biol. 1978;42 Pt 1:439-47 PMID: 209942
  25. P1 plasmid replication: replicon structure.
    J Mol Biol. 1984 Mar 5;173(3):307-24 PMID: 6699914
  26. Mini-F plasmid genes that couple host cell division to plasmid proliferation.
    Proc Natl Acad Sci U S A. 1983 Aug;80(15):4784-8 PMID: 6308648
  27. Effects of the ccd function of the F plasmid on bacterial growth.
    J Bacteriol. 1985 Sep;163(3):841-9 PMID: 3897195
  28. Isolation and characterization of nucleoid proteins from Escherichia coli.
    Mol Gen Genet. 1984;196(2):217-24 PMID: 6387397
  29. Initiation of enzymatic replication at the origin of the Escherichia coli chromosome: contributions of RNA polymerase and primase.
    Proc Natl Acad Sci U S A. 1985 Jun;82(11):3562-6 PMID: 2987933
  30. Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.
    Gene. 1977;2(2):95-113 PMID: 344137
  31. Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid.
    J Bacteriol. 1978 Jun;134(3):1141-56 PMID: 149110
  32. Molecular cloning and nucleotide sequence of the HU-1 gene of Escherichia coli.
    Mol Gen Genet. 1985;201(2):360-2 PMID: 3003540
  33. Protein HU in the enzymatic replication of the chromosomal origin of Escherichia coli.
    Proc Natl Acad Sci U S A. 1984 Jan;81(2):424-8 PMID: 6364143
  34. Control of F plasmid replication by a host gene: evidence for interaction of the mafA gene product of Escherichia coli with the mini-F incC region.
    J Bacteriol. 1984 Dec;160(3):1130-6 PMID: 6094502
  35. Participation of the hup gene product in site-specific DNA inversion in Escherichia coli.
    Gene. 1989;76(2):345-52 PMID: 2666260
  36. High-copy-number and low-copy-number plasmid vectors for lacZ alpha-complementation and chloramphenicol- or kanamycin-resistance selection.
    Gene. 1987;61(1):63-74 PMID: 3327753
  37. Replication of Fpoh+ plasmid in mafA mutants of Escherichia coli defective in plasmid maintenance.
    Mol Gen Genet. 1977 Apr 29;152(3):211-7 PMID: 327277
  38. Structure and properties of the bacterial nucleoid.
    Cell. 1982 Oct;30(3):667-9 PMID: 6291779
  39. Complete amino-acid sequences of DNA-binding proteins HU-1 and HU-2 from Escherichia coli.
    Eur J Biochem. 1980 Feb;103(3):447-61 PMID: 6987059
  40. Chromosomal genes essential for stable maintenance of the mini-F plasmid in Escherichia coli.
    J Bacteriol. 1988 Nov;170(11):5272-8 PMID: 3053654
  41. The integration host factor of Escherichia coli binds to bent DNA at the origin of replication of the plasmid pSC101.
    Cell. 1987 Jun 5;49(5):709-17 PMID: 3555843
  42. Cloning and sequencing of the HU-2 gene of Escherichia coli.
    Mol Gen Genet. 1987 Sep;209(2):408-10 PMID: 3312963
  43. Involvement of DnaK protein in mini-F plasmid replication: temperature-sensitive seg mutations are located in the dnaK gene.
    Mol Gen Genet. 1989 Aug;218(2):183-9 PMID: 2674651
  44. Host participation in plasmid maintenance: dependence upon dnaA of replicons derived from P1 and F.
    Proc Natl Acad Sci U S A. 1986 Jun;83(12):4423-7 PMID: 3520571
Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00260482
Published
1990-01-00
Pages
197-203
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00260482
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