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PMID: 223159 Published · ppublish English Journal Article

Sites of sequence variability in Epstein-Barr virus DNA from different sources.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 76 ·No. 6 ·1979-06-00 ·Pages 2794-8

Rymo L, Lindahl T, Adams A

Abstract

The intracellular Epstein-Barr virus (EBV) DNA present in virus-transformed cells was partly purified from 23 cell lines or biopsies of Burkitt lymphoma, nasopharyngeal carcinoma, infectious mononucleosis, or healthy carrier origin. Such DNA was cleaved in fragments (A-K) of molecular weights between 1 x 10(6) and 30 x 10(6) with restriction enzyme EcoRI, and these fragments were analyzed by standard methods involving agarose gel electrophoresis, transfer to nitrocellulose filters, and hybridization with radioactive EBV DNA or complementary RNA. Sequence variability among different EBV DNA isolates was largely confined to the A, C, and I fragments. These results are discussed in relation to the linkage map of the EcoRI fragments of EBV DNA. The EcoRI cleavage pattern of intracellular viral DNA of an EBV-like virus from baboon cells, Herpesvirus papio, was entirely different from that of human EBV isolates.

MeSH Terms
Animals Base Sequence Cell Line Cell Transformation, Viral DNA Restriction Enzymes DNA, Viral Herpesvirus 4, Human/analysis Mice
Chemicals
DNA, Viral DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Rymo L
Lindahl T
Adams A
References (26)
26 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.76.6.2794
Published
1979-06-00
Pages
2794-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC383695
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.76.6.2794 PMC Full Text View journal details
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