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PMID: 2228962 Published · ppublish English Comparative Study Journal Article

Cloning, mapping, and sequencing of the gene encoding Escherichia coli quinoprotein glucose dehydrogenase.

Journal of bacteriology ·Vol. 172 ·No. 11 ·1990-11-00 ·Pages 6308-15

Cleton-Jansen AM, Goosen N, Fayet O, van de Putte P

Abstract

Escherichia coli contains pyrroloquinoline quinone-dependent glucose dehydrogenase. We cloned and sequenced the gene (gcd) encoding this enzyme and showed that the derived amino acid sequence is highly homologous to that of the gdhA gene product of Acinetobacter calcoaceticus. Stretches of homology also exist between the amino acid sequence of E. coli glucose dehydrogenase and other pyrroloquinoline quinone-dependent dehydrogenases from several bacterial species. The position of gcd on the chromosomal map of E. coli was determined to be at 3.1 min.

Related Genes
gcd
MeSH Terms
Amino Acid Sequence Base Sequence Blotting, Southern Chromosomes, Bacterial Cloning, Molecular/methods DNA, Bacterial/genetics,isolation & purification Escherichia coli/enzymology,genetics Genes, Bacterial Genetic Complementation Test Genomic Library Glucose Dehydrogenases/genetics Molecular Sequence Data Plasmids Restriction Mapping Sequence Homology, Nucleic Acid
Chemicals
DNA, Bacterial Glucose Dehydrogenases glucose dehydrogenase (pyrroloquinoline-quinone)
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Cleton-Jansen A M
Laboratory of Molecular Genetics, University of Leiden, The Netherlands.
Goosen N
Fayet O
van de Putte P
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.172.11.6308-6315.1990
Published
1990-11-00
Pages
6308-15
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC526814
Subset
IM
Databases
GENBANK
X51323
External Links
PubMed source DOI: 10.1128/jb.172.11.6308-6315.1990 PMC Full Text View journal details
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