Home LiteratureArticle Details

PMID: 2834325 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Cloning of the gene encoding quinoprotein glucose dehydrogenase from Acinetobacter calcoaceticus: evidence for the presence of a second enzyme.

Journal of bacteriology ·Vol. 170 ·No. 5 ·1988-05-00 ·Pages 2121-5

Cleton-Jansen AM, Goosen N, Wenzel TJ, van de Putte P

Abstract

We cloned the gene coding for the quinoprotein glucose dehydrogenase from Acinetobacter calcoaceticus. This clone complements gdh mutations in A. calcoaceticus, Pseudomonas aeruginosa, and Escherichia coli. The gene codes for a protein with an Mr of 83,000. Evidence is presented for the presence of two different glucose dehydrogenase enzymes in A. calcoaceticus: a protein with an Mr of 83,000 and a dimer of two identical subunits with an Mr of 50,000.

MeSH Terms

Acinetobacter/enzymology,genetics Carbohydrate Dehydrogenases/genetics Chromosome Mapping Cloning, Molecular DNA Restriction Enzymes Escherichia coli/enzymology,genetics,growth & development Gene Expression Regulation Genes, Bacterial Genetic Complementation Test Glucose Dehydrogenases/genetics Mutation Plasmids Pseudomonas/enzymology,genetics Pseudomonas aeruginosa/enzymology,genetics

Chemicals

Carbohydrate Dehydrogenases Glucose Dehydrogenases glucose dehydrogenase (pyrroloquinoline-quinone) DNA Restriction Enzymes

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Cleton-Jansen A M

Laboratory of Molecular Genetics, University of Leiden, The Netherlands.

Goosen N

Wenzel T J

van de Putte P

References (10)

10 references, click to expand

Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
The regulation of transport of glucose and methyl alpha-glucoside in Pseudomonas aeruginosa.
Biochem J. 1973 Feb;132(2):141-54 PMID: 4199012
Glucose dehydrogenase from Acinetobacter calcoaceticus: a 'quinoprotein'.
FEBS Lett. 1979 Dec 15;108(2):443-6 PMID: 520586
A system for shotgun DNA sequencing.
Nucleic Acids Res. 1981 Jan 24;9(2):309-21 PMID: 6259625
Purification and characterization of quinoprotein glucose dehydrogenase from Acinetobacter calcoaceticus L.M.D. 79.41.
Biochem J. 1986 Oct 1;239(1):163-7 PMID: 3800975
Cloning of human purine-nucleoside phosphorylase cDNA sequences by complementation in Escherichia coli.
Proc Natl Acad Sci U S A. 1983 Jul;80(14):4281-5 PMID: 6410388
Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors.
Gene. 1985;33(1):103-19 PMID: 2985470
Energy transduction by electron transfer via a pyrrolo-quinoline quinone-dependent glucose dehydrogenase in Escherichia coli, Pseudomonas aeruginosa, and Acinetobacter calcoaceticus (var. lwoffi).
J Bacteriol. 1985 Aug;163(2):493-9 PMID: 3926746
Cloning of the genes involved in synthesis of coenzyme pyrrolo-quinoline-quinone from Acinetobacter calcoaceticus.
J Bacteriol. 1987 Jan;169(1):303-7 PMID: 3539922
Broad host range DNA cloning system for gram-negative bacteria: construction of a gene bank of Rhizobium meliloti.
Proc Natl Acad Sci U S A. 1980 Dec;77(12):7347-51 PMID: 7012838

Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.170.5.2121-2125.1988

Published

1988-05-00

Pages

2121-5

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC211095

Subset

External Links

PubMed source DOI: 10.1128/jb.170.5.2121-2125.1988 PMC Full Text View journal details

Analysis Services

Cloning of the gene encoding quinoprotein glucose dehydrogenase from Acinetobacter calcoaceticus: evidence for the presence of a second enzyme.

Contact

WeChat Official Account