Cloning and sequencing of the gene encoding the 72-kilodalton dehydrogenase subunit of alcohol dehydrogenase from Acetobacter aceti.
A genomic library of Acetobacter aceti DNA was constructed by using a broad-host-range cosmid vector. Complementation of a spontaneous alcohol dehydrogenase-deficient mutant resulted in the isolation of a plasmid designated pAA701. Subcloning and deletion analysis of pAA701 limited the region that complemented the deficiency in alcohol dehydrogenase activity of the mutant. The nucleotide sequence of this region was determined and showed that this region contained the full structural gene for the 72-kilodalton dehydrogenase subunit of the alcohol dehydrogenase enzyme complex. The predicted amino acid sequence of the gene showed homology with sequences of methanol dehydrogenase structural genes of Paracoccus denitrificans and Methylobacterium organophilum.
17 references, click to expand
-
Enzymology of quinoproteins.Adv Enzymol Relat Areas Mol Biol. 1987;59:169-212 PMID: 3544710
-
Bacterial oxidation of methane and methanol.Adv Microb Physiol. 1986;27:113-210 PMID: 3020939
-
Isolation and nucleotide sequence of the methanol dehydrogenase structural gene from Paracoccus denitrificans.J Bacteriol. 1987 Sep;169(9):3969-75 PMID: 3114231
-
Identification of putative methanol dehydrogenase (moxF) structural genes in methylotrophs and cloning of moxF genes from Methylococcus capsulatus bath and Methylomonas albus BG8.J Bacteriol. 1988 May;170(5):2063-9 PMID: 3129400
-
Nucleotide sequence and transcriptional start site of the Methylobacterium organophilum XX methanol dehydrogenase structural gene.J Bacteriol. 1988 Oct;170(10):4739-47 PMID: 2459109
-
Determinant of cistron specificity in bacterial ribosomes.Nature. 1975 Mar 6;254(5495):34-8 PMID: 803646
-
Alcohol dehydrogenase gene from Alcaligenes eutrophus: subcloning, heterologous expression in Escherichia coli, sequencing, and location of Tn5 insertions.J Bacteriol. 1988 Nov;170(11):5248-56 PMID: 2846513
-
DNA sequencing with chain-terminating inhibitors.Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
-
Replication of an origin-containing derivative of plasmid RK2 dependent on a plasmid function provided in trans.Proc Natl Acad Sci U S A. 1979 Apr;76(4):1648-52 PMID: 377280
-
The use of synthetic oligonucleotides as hybridization probes. II. Hybridization of oligonucleotides of mixed sequence to rabbit beta-globin DNA.Nucleic Acids Res. 1981 Feb 25;9(4):879-94 PMID: 7232206
-
Cloning of genes controlling alginate biosynthesis from a mucoid cystic fibrosis isolate of Pseudomonas aeruginosa.J Bacteriol. 1984 Jul;159(1):9-18 PMID: 6330052
-
Quinoprotein alcohol dehydrogenase from ethanol-grown Pseudomonas aeruginosa.Biochem J. 1984 Nov 1;223(3):921-4 PMID: 6439190
-
Signal sequences. The limits of variation.J Mol Biol. 1985 Jul 5;184(1):99-105 PMID: 4032478
-
Protein secretion in Escherichia coli.Annu Rev Microbiol. 1985;39:615-48 PMID: 3904614
-
Conjugative transfer of the naturally occurring plasmids of Acetobacter xylinum by IncP-plasmid-mediated mobilization.J Bacteriol. 1986 Jan;165(1):336-9 PMID: 3001030
-
Quinohaemoprotein alcohol dehydrogenase apoenzyme from Pseudomonas testosteroni.Biochem J. 1986 Mar 15;234(3):611-5 PMID: 3521592
-
Cloning and sequencing of the alcohol dehydrogenase II gene from Zymomonas mobilis.J Bacteriol. 1987 Jun;169(6):2591-7 PMID: 3584063
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