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PMID: 22258242 Published · ppublish English Journal Article Research Support, N.I.H., Extramural

Protruding domain of capsid protein is necessary and sufficient to determine murine norovirus replication and pathogenesis in vivo.

Journal of virology ·Vol. 86 ·No. 6 ·2012-03-00 ·Pages 2950-8

Strong DW, Thackray LB, Smith TJ, Virgin HW

Abstract

Human noroviruses (HuNoVs) are the major cause of epidemic, nonbacterial gastroenteritis worldwide. Due to the lack of a tractable model system and the inability to grow HuNoVs in cell culture, factors required for the norovirus (NoV) life cycle and pathogenesis in the host remain largely unknown. The discovery of murine norovirus (MNV) and the development of reverse-genetics systems for this virus provide an opportunity to study these aspects of NoV infection in vitro and in vivo. Previous studies identified a single amino acid at residue 296 in the protruding (P) domain of the capsid protein that is responsible for determining the virulence of the MNV clone MNV1.CW1 in 12956/SvEv background STAT1-deficient (STAT1(-/-)) mice. In this report, we identified and characterized another determinant of lethality in the P domain that is necessary and sufficient to determine the replication and pathogenesis of the MNV clones MNV1.CW3 and CR6.STL1 in C57BL/6 background STAT1(-/-) mice. Furthermore, we describe how the role of residue 296 in MNV virulence differs between STAT1(-/-) mouse strains. We also describe potential interactions between subdomains of the P domain, as well as between other virus elements, which facilitate recovery of MNV using a reverse-genetics system.

MeSH Terms
Animals Caliciviridae Infections/virology Capsid Proteins/chemistry,genetics,metabolism Cell Line Mice Mice, Inbred C57BL Mice, Knockout Molecular Sequence Data Norovirus/chemistry,genetics,pathogenicity,physiology Protein Structure, Tertiary Virulence Virus Replication
Chemicals
Capsid Proteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Strong David W
Department of Pathology and Immunology, Washington University School of Medicine, St. Louis, Missouri, USA.
Thackray Larissa B
Smith Tom J
Virgin Herbert W
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
1098-5514
DOI
10.1128/JVI.07038-11
Published
2012-03-00
Epub
2012-00-18
Pages
2950-8
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC3302348
Subset
IM
Grants
NIAID NIH HHS · R01 AI054483 · United States
PHS HHS · 5T32A100716334 · United States
NIAID NIH HHS · AI0544483 · United States
Databases
GENBANK
JQ237823
External Links
PubMed source DOI: 10.1128/JVI.07038-11 PMC Full Text View journal details
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