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PMID: 22018636 Published · ppublish English Comparative Study Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Comparative murine norovirus studies reveal a lack of correlation between intestinal virus titers and enteric pathology.

Virology ·Vol. 421 ·No. 2 ·2011-12-20 ·Pages 202-10

Kahan SM, Liu G, Reinhard MK, Hsu CC, Livingston RS, Karst SM

Abstract

Human noroviruses are significant emerging pathogens, causing the majority of non-bacterial gastroenteritis outbreaks worldwide. The recent discovery of 30 murine norovirus strains is beginning to facilitate a detailed investigation of norovirus pathogenesis. Here, we have performed an in vivo comparative analysis of two murine norovirus strains, MNV-1 and MNV-3. In immunocompetent mice, MNV-1 caused modest intestinal pathology whereas MNV-3 was attenuated compared to MNV-1. Surprisingly though, MNV-3 reached higher titers in intestinal tissue than MNV-1. MNV-3 also displayed attenuation in mice deficient in the critical interferon signaling molecule STAT-1, demonstrating that MNV-3 attenuation is not a result of increased interferon sensitivity. Importantly, MNV-3-infected mice lost weight and developed gastric bloating and diarrhea in STAT1(-/-) mice, from which all animals recovered. This disease profile recapitulates several key features of acute gastroenteritis experienced by people infected with a human norovirus.

MeSH Terms
Animals Caliciviridae Infections/immunology,pathology,virology Cell Line Gastroenteritis/immunology,pathology,virology Interferons/immunology Mice Mice, Inbred C3H Mice, Knockout Norovirus/growth & development,immunology,pathogenicity STAT1 Transcription Factor/deficiency,genetics,metabolism Viral Load Virus Replication
Chemicals
STAT1 Transcription Factor Stat1 protein, mouse Interferons
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Kahan Shannon M
College of Medicine, Department of Molecular Genetics and Microbiology, University of Florida, Gainesville, FL, USA.
Liu Guangliang
Reinhard Mary K
Hsu Charlie C
Livingston Robert S
Karst Stephanie M
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Article Info
Journal
Virology
Abbr.
Virology
ISSN
1096-0341
DOI
10.1016/j.virol.2011.09.030
Published
2011-12-20
Epub
2011-00-22
Pages
202-10
Language
English
Region
United States
NLM ID
0110674
PMCID
PMC3210872
Subset
IM
Grants
NIAID NIH HHS · AI082310 · United States
NIAID NIH HHS · AI081921 · United States
NIAID NIH HHS · R01 AI081921 · United States
NCRR NIH HHS · P20 RR018724 · United States
NIAID NIH HHS · R01 AI081921-02 · United States
NCRR NIH HHS · P20-RR018724 · United States
NIAID NIH HHS · R21 AI082310-02 · United States
NIAID NIH HHS · R56 AI081921 · United States
NIAID NIH HHS · R21 AI082310 · United States
External Links
PubMed source DOI: 10.1016/j.virol.2011.09.030 PMC Full Text
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