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PMID: 2196444 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Regulation of the Discoidin I gamma gene in Dictyostelium discoideum: identification of individual promoter elements mediating induction of transcription and repression by cyclic AMP.

Molecular and cellular biology ·Vol. 10 ·No. 8 ·1990-08-00 ·Pages 4080-8

Vauti F, Morandini P, Blusch J, Sachse A, Nellen W

Abstract

We dissected the promoter of the developmentally induced and cyclic AMP-repressed discoidin I gamma gene and identified a sequence element essential for developmental induction. Transfer of the element to an inactive heterologous promoter demonstrated that this sequence is sufficient to confer expression in axenically growing cells and to induce gene activity in development after growth on bacteria. A 16-base-pair sequence within this element was shown to be sufficient for induction in the discoidin promoter context and was used to reactivate different truncated promoter constructs. This led to the localization of an element necessary for down regulation of gene expression by extracellular cyclic AMP.

MeSH Terms
Base Sequence Carrier Proteins/genetics Chloramphenicol O-Acetyltransferase/genetics Chromosome Deletion Dictyostelium/genetics Discoidins Escherichia coli/genetics Fungal Proteins/genetics Gene Expression Gene Expression Regulation, Fungal Genes, Fungal Lectins Molecular Sequence Data Oligonucleotide Probes Promoter Regions, Genetic Protozoan Proteins
Chemicals
Carrier Proteins Discoidins Fungal Proteins Lectins Oligonucleotide Probes Protozoan Proteins Chloramphenicol O-Acetyltransferase
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Vauti F
Max-Planck-Institut für Biochemie, Abt. Zellbiologie, Martinsried, Federal Republic of Germany.
Morandini P
Blusch J
Sachse A
Nellen W
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36 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.10.8.4080-4088.1990
Published
1990-08-00
Pages
4080-8
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC360925
Subset
IM
Databases
GENBANK
M55332
External Links
PubMed source DOI: 10.1128/mcb.10.8.4080-4088.1990 PMC Full Text View journal details
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