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PMID: 3600646 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Developmental regulation of DNase I-hypersensitive sites in Dictyostelium discoideum.

Molecular and cellular biology ·Vol. 7 ·No. 5 ·1987-05-00 ·Pages 1823-9

Ayres K, Neuman W, Rowekamp WG, Chung S

Abstract

We have studied two regions of Dictyostelium discoideum chromatin and identified several DNase I-hypersensitive sites in these regions. One of these sites is located about 300 to 500 bases upstream of the transcriptional start site of a gene that is expressed at all stages of development. This site is present in both vegetative cells and postaggregation cells. Another hypersensitive site is associated with a gene that is expressed only after the multicellular stage. This site is located about 400 bases upstream of the start site, and it is present only in postaggregation cells. Thus, much like higher eucaryotes, D. discoideum contains DNase I-hypersensitive sites that may be involved in the regulation of the genes with which they are associated.

MeSH Terms
Cell Differentiation Chromatin/ultrastructure Chromosome Mapping DNA, Fungal/genetics Deoxyribonuclease I Dictyostelium/genetics,growth & development Gene Expression Regulation Promoter Regions, Genetic RNA, Messenger/genetics Transcription, Genetic
Chemicals
Chromatin DNA, Fungal RNA, Messenger Deoxyribonuclease I
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Ayres K
Neuman W
Rowekamp W G
Chung S
References (29)
29 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.7.5.1823-1829.1987
Published
1987-05-00
Pages
1823-9
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC365285
Subset
IM
Grants
NIGMS NIH HHS · GM 31226 · United States
Databases
GENBANK
M16345, M16346
External Links
PubMed source DOI: 10.1128/mcb.7.5.1823-1829.1987 PMC Full Text View journal details
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