Home LiteratureArticle Details
PMID: 3024117 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Hypersensitive sites in the 5' and 3' flanking regions of the cysteine proteinase I gene of Dictyostelium discoideum.

Nucleic acids research ·Vol. 14 ·No. 22 ·1986-11-25 ·Pages 8703-22

Pavlovic J, Banz E, Parish RW

Abstract

The cysteine proteinase I gene of Dictyostelium discoideum is a developmentally regulated single copy gene. Specific sites in the 5' and the 3' flanking regions of the gene were cleaved by an endogenous nuclease when the gene was being transcribed. The majority of these sites were not cut when the gene was inactive. A dramatic change in the pattern of micrococcal nuclease and DNase I hypersensitive sites occurred in the 5' flanking region when transcription commenced at the 8 h stage of development. The major sites, doublets at -220/-300 bp and -670/-770 bp upstream of the transcription start site, corresponded to those cut by the endogenous nuclease. When transcription subsequently ceased the hypersensitive sites did not significantly change, indicating the gene remained in an activated state. The micrococcal nuclease hypersensitive sites in the 3' flanking region did not change significantly during development.

MeSH Terms
Base Sequence Cysteine Endopeptidases DNA Restriction Enzymes Deoxyribonuclease I Dictyostelium/enzymology,genetics Endopeptidases/genetics Genes Genes, Fungal Genes, Regulator Micrococcal Nuclease Nucleic Acid Hybridization Transcription, Genetic
Chemicals
DNA Restriction Enzymes Deoxyribonuclease I Micrococcal Nuclease Endopeptidases Cysteine Endopeptidases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Pavlovic J
Banz E
Parish R W
References (42)
42 references, click to expand
  1. Control of enzyme synthesis by cellular interaction during development of the cellular slime mold Dictyostelium discoideum.
    J Mol Biol. 1971 Jun 14;58(2):541-54 PMID: 4103925
  2. Morphogenesis in the slime mold Dictyostelium discoideum. 1. The accumulation and erasure of "morphogenetic information".
    Dev Biol. 1975 Dec;47(2):292-302 PMID: 1239391
  3. Resynthesis of developmentally regulated plasma membrane proteins following disaggregation of Dictyostelium pseudoplasmodia.
    FEBS Lett. 1979 Mar 15;99(2):270-4 PMID: 428552
  4. Biochemical evidence for a distinctive chromatin structure in nucleoli of Dictyostelium.
    FEBS Lett. 1979 Oct 15;106(2):363-9 PMID: 499521
  5. Non-random cleavage of SV40 DNA in the compact minichromosome and free in solution by micrococcal nuclease.
    Biochem Biophys Res Commun. 1980 Jan 29;92(2):532-9 PMID: 6243943
  6. Hb switching in chickens.
    Cell. 1980 Apr;19(4):973-80 PMID: 6155217
  7. Absence of nucleosomes in a fraction of SV40 chromatin between the origin of replication and the region coding for the late leader RNA.
    Cell. 1980 May;20(1):65-73 PMID: 6248237
  8. Tissue-specific DNA cleavages in the globin chromatin domain introduced by DNAase I.
    Cell. 1980 Jun;20(2):451-60 PMID: 7388947
  9. A 200 base pair region at the 5' end of the chicken adult beta-globin gene is accessible to nuclease digestion.
    Cell. 1981 Nov;27(1 Pt 2):45-55 PMID: 6276024
  10. Micrococcal nuclease as a probe of DNA sequence organization and chromatin structure.
    Cell. 1981 Nov;27(1 Pt 2):57-64 PMID: 6799212
  11. Transcriptional control signals of a eukaryotic protein-coding gene.
    Science. 1982 Jul 23;217(4557):316-24 PMID: 6283634
  12. Differences in the nuclease sensitivity between the two alleles of the immunoglobulin kappa light chain genes in mouse liver and myeloma nuclei.
    Nucleic Acids Res. 1982 Jun 25;10(12):3627-45 PMID: 6287416
  13. A complex of interacting DNAase I-hypersensitive sites near the Drosophila glue protein gene, Sgs4.
    Cell. 1982 Jun;29(2):601-7 PMID: 6214314
  14. Chromatin changes accompany immunoglobulin kappa gene activation: a potential control region within the gene.
    Nature. 1982 Sep 30;299(5882):449-51 PMID: 6811947
  15. The structure of the thymidine kinase gene promoter: nuclease hypersensitivity correlates with expression.
    Cell. 1982 Dec;31(2 Pt 1):347-53 PMID: 6297761
  16. Chromatin fine structure of the histone gene complex of Drosophila melanogaster.
    Nucleic Acids Res. 1983 Jan 25;11(2):421-39 PMID: 6402757
  17. DNase I-hypersensitive sites in the chromatin of immunoglobulin kappa light chain genes.
    Proc Natl Acad Sci U S A. 1983 May;80(9):2427-31 PMID: 6221340
  18. Chromatin structure along the ribosomal DNA of Dictyostelium. Regional differences and changes accompanying cell differentiation.
    J Mol Biol. 1983 May 25;166(3):361-81 PMID: 6304325
  19. Nuclease-hypersensitive sites in the chromatin domain of the chicken lysozyme gene.
    Nucleic Acids Res. 1983 Jun 11;11(11):3467-85 PMID: 6304632
  20. Structure and function of the promoter-enhancer region of polyoma and SV40.
    Cold Spring Harb Symp Quant Biol. 1983;47 Pt 2:935-44 PMID: 6305593
  21. A tissue-specific transcription enhancer element is located in the major intron of a rearranged immunoglobulin heavy chain gene.
    Cell. 1983 Jul;33(3):717-28 PMID: 6409417
  22. A lymphocyte-specific cellular enhancer is located downstream of the joining region in immunoglobulin heavy chain genes.
    Cell. 1983 Jul;33(3):729-40 PMID: 6409418
  23. Immunoglobulin gene transcription is activated by downstream sequence elements.
    Cell. 1983 Jul;33(3):741-8 PMID: 6409419
  24. DNase I hypersensitive sites in the chromatin of human mu immunoglobulin heavy-chain genes.
    Nature. 1983 Dec 22-1984 Jan 4;306(5945):809-12 PMID: 6419125
  25. Chromatin organization of the 87A7 heat shock locus of Drosophila melanogaster.
    J Mol Biol. 1984 Feb 5;172(4):385-403 PMID: 6319717
  26. Induction of altered chromatin structures by simian virus 40 enhancer and promoter elements.
    Nature. 1984 Feb 23-29;307(5953):708-14 PMID: 6321997
  27. Two protein-binding sites in chromatin implicated in the activation of heat-shock genes.
    Nature. 1984 May 17-23;309(5965):229-34 PMID: 6325944
  28. A close association between sites of DNase I hypersensitivity and sites of enhanced cleavage by micrococcal nuclease in the 5'-flanking region of the actively transcribed ovalbumin gene.
    EMBO J. 1984 May;3(5):1137-44 PMID: 6329739
  29. Temporal order of chromatin structural changes associated with activation of the major chicken vitellogenin gene.
    Cell. 1983 May;33(1):65-76 PMID: 6088056
  30. Histone-H1-dependent chromatin superstructures and the suppression of gene activity.
    Cell. 1984 Aug;38(1):17-27 PMID: 6467367
  31. Correlation between DNase I hypersensitive sites and putative regulatory sequences in human immunoglobulin genes of the kappa light chain type.
    Nucleic Acids Res. 1984 Sep 25;12(18):7007-21 PMID: 6091050
  32. Chromatin structure at the 44D larval cuticle gene locus in Drosophila: the effect of a transposable element insertion.
    Nucleic Acids Res. 1984 Dec 11;12(23):9025-38 PMID: 6096816
  33. Chemical footprinting of 5S RNA chromatin in embryos of Drosophila melanogaster.
    EMBO J. 1984 Dec 20;3(13):3101-8 PMID: 6084593
  34. A developmentally regulated cysteine proteinase in Dictyostelium discoideum.
    EMBO J. 1985 Apr;4(4):999-1006 PMID: 2990918
  35. Characterization of two highly diverged but developmentally co-regulated cysteine proteinase genes in Dictyostelium discoideum.
    Nucleic Acids Res. 1985 Dec 20;13(24):8853-66 PMID: 3909109
  36. Mapping of endogenous nuclease-sensitive regions and of putative topoisomerase sites of action along the chromatin of Dictyostelium ribosomal RNA genes.
    J Mol Biol. 1986 Apr 5;188(3):287-300 PMID: 3016283
  37. DNAase I-hypersensitive sites of chromatin.
    Cell. 1981 Dec;27(3 Pt 2):413-5 PMID: 6101195
  38. The 5' ends of Drosophila heat shock genes in chromatin are hypersensitive to DNase I.
    Nature. 1980 Aug 28;286(5776):854-60 PMID: 6774262
  39. Chromatin structure of the histone genes of D. melanogaster.
    Cell. 1981 Feb;23(2):401-9 PMID: 6258802
  40. A change in the rate of transcription of a eukaryotic gene in response to cyclic AMP.
    Proc Natl Acad Sci U S A. 1980 Dec;77(12):7171-5 PMID: 6261244
  41. Sequence specific cleavage of DNA by micrococcal nuclease.
    Nucleic Acids Res. 1981 Jun 25;9(12):2643-58 PMID: 7279658
  42. High sequence specificity of micrococcal nuclease.
    Nucleic Acids Res. 1981 Jun 25;9(12):2659-73 PMID: 6269057
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/14.22.8703
Published
1986-11-25
Pages
8703-22
Language
English
Region
England
NLM ID
0411011
PMCID
PMC311906
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/14.22.8703 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2