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PMID: 6091050 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Correlation between DNase I hypersensitive sites and putative regulatory sequences in human immunoglobulin genes of the kappa light chain type.

Nucleic acids research ·Vol. 12 ·No. 18 ·1984-09-25 ·Pages 7007-21

Pospelov VA, Klobeck HG, Zachau HG

Abstract

The human lymphoid cell lines Walker and Daudi constitute a particularly suitable system for studies on the chromatin structure of K light chain genes (see preceding paper). The rearranged and non-rearranged alleles of Walker cells were found to be about equally sensitive towards digestion with DNAase I. A DNAase I hypersensitive site was mapped 0.13 kb upstream of the leader segment of the rearranged VK genes; it comprises a region in which promoter-like regulatory elements were discovered recently. Additional hypersensitive sites are located further upstream. A hypersensitive site in the JK-CK intron coincides with a putative tissue specific enhancer element. A hypersensitive region down-stream of CK overlaps with the cleavage/polyadenylation recognition signal which is flanked by sequences related to the above mentioned putative regulatory sequences. The coincidence between DNAase I hypersensitive sites and those sequences may be functionally significant.

MeSH Terms
Base Sequence Burkitt Lymphoma/immunology Cell Line Cell Nucleus/analysis DNA Restriction Enzymes DNA, Neoplasm/isolation & purification Deoxyribonuclease I/metabolism Genes HeLa Cells/immunology Humans Immunoglobulin Light Chains/genetics Immunoglobulin kappa-Chains/genetics Nucleic Acid Hybridization
Chemicals
DNA, Neoplasm Immunoglobulin Light Chains Immunoglobulin kappa-Chains DNA Restriction Enzymes Deoxyribonuclease I
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Pospelov V A
Klobeck H G
Zachau H G
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25 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/12.18.7007
Published
1984-09-25
Pages
7007-21
Language
English
Region
England
NLM ID
0411011
PMCID
PMC320139
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/12.18.7007 PMC Full Text View journal details
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