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PMID: 2190990 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Centrosome duplication continues in cycloheximide-treated Xenopus blastulae in the absence of a detectable cell cycle.

The Journal of cell biology ·Vol. 110 ·No. 6 ·1990-06-00 ·Pages 2033-42

Gard DL, Hafezi S, Zhang T, Doxsey SJ

Abstract

Cycloheximide (500 micrograms/ml) rapidly arrests cleavage, spindle assembly, and cycles of an M-phase-specific histone kinase in early Xenopus blastulae. 2 h after cycloheximide addition, most cells contained two microtubule asters radiating from perinuclear microtubule organizing centers (MTOCs). In contrast, blastomeres treated with cycloheximide for longer periods (3-6 h) contained numerous microtubule asters and MTOCs. Immunofluorescence with an anticentrosome serum and EM demonstrated that the MTOCs in cycloheximide-treated cells were typical centrosomes, containing centrioles and pericentriolar material. We conclude that centrosome duplication continues in cycloheximide-treated Xenopus blastulae in the absence of a detectable cell cycle. In addition, these observations suggest that Xenopus embryos contain sufficient material to assemble 1,000-2,000 centrosomes in the absence of normal protein synthesis.

MeSH Terms
Animals Blastocyst/cytology,drug effects,ultrastructure Cell Cycle/drug effects,physiology Centrioles/drug effects,physiology Cycloheximide/pharmacology Fluorescent Antibody Technique Interphase Microtubules/drug effects,physiology,ultrastructure Spindle Apparatus/drug effects,physiology Time Factors Xenopus laevis/embryology
Chemicals
Cycloheximide
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Gard D L
Department of Biology, University of Utah, Salt Lake City.
Hafezi S
Zhang T
Doxsey S J
References (42)
42 references, click to expand
  1. The centriole cycle in synchronized HeLa cells.
    J Cell Biol. 1968 Feb;36(2):329-39 PMID: 5638885
  2. In vitro polymerization of microtubules into asters and spindles in homogenates of surf clam eggs.
    J Cell Biol. 1975 Jan;64(1):146-58 PMID: 45844
  3. Dependence of centriole formation on protein synthesis.
    J Cell Biol. 1976 Jul;70(1):9-19 PMID: 932104
  4. The synthesis and storage of histones during the oogenesis of Xenopus laevis.
    Dev Biol. 1977 May;57(1):118-35 PMID: 558925
  5. Studies on the de novo formation of centrioles: aster formation in the activated eggs of sea urchin.
    J Cell Sci. 1977 Apr;24:203-16 PMID: 893543
  6. Multiple sites for the initiation of microtubule assembly in mammalian cells.
    Cell. 1979 Feb;16(2):239-52 PMID: 455434
  7. A cytoplasmic clock with the same period as the division cycle in Xenopus eggs.
    Proc Natl Acad Sci U S A. 1980 Jan;77(1):462-6 PMID: 6928638
  8. Achromosomal cleavage of fertilized starfish eggs in the presence of aphidicolin.
    Dev Biol. 1981 Jul 30;85(2):409-15 PMID: 6790320
  9. Tubulin assembly sites and the organization of cytoplasmic microtubules in cultured mammalian cells.
    J Cell Biol. 1981 Sep;90(3):554-62 PMID: 7026576
  10. The reproduction of centrosomes: nuclear versus cytoplasmic controls.
    J Cell Biol. 1986 Nov;103(5):1873-81 PMID: 3782286
  11. The presence of centrioles in artificially activated sea urchin eggs.
    J Biophys Biochem Cytol. 1961 Oct;11:244-7 PMID: 13886557
  12. Centriole cycle in Chinese hamster ovary cells as determined by whole-mount electron microscopy.
    J Cell Biol. 1981 Dec;91(3 Pt 1):814-21 PMID: 7328123
  13. Centrioles in the cell cycle. I. Epithelial cells.
    J Cell Biol. 1982 Jun;93(3):938-49 PMID: 7119006
  14. A major developmental transition in early Xenopus embryos: I. characterization and timing of cellular changes at the midblastula stage.
    Cell. 1982 Oct;30(3):675-86 PMID: 6183003
  15. Continuous hypertonic conditions activate and promote the formation of new centrioles within cytasters in sea urchin eggs.
    Cell Biol Int Rep. 1982 Nov;6(11):1025-31 PMID: 6890880
  16. Anticentromere and anticentriole antibodies in the scleroderma spectrum.
    Arch Dermatol. 1983 Jul;119(7):560-6 PMID: 6859899
  17. Cyclin: a protein specified by maternal mRNA in sea urchin eggs that is destroyed at each cleavage division.
    Cell. 1983 Jun;33(2):389-96 PMID: 6134587
  18. Maturation-promoting factor induces nuclear envelope breakdown in cycloheximide-arrested embryos of Xenopus laevis.
    J Cell Biol. 1983 Jul;97(1):81-91 PMID: 6345556
  19. Spontaneous formation of nucleus-like structures around bacteriophage DNA microinjected into Xenopus eggs.
    Cell. 1983 Aug;34(1):13-23 PMID: 6224569
  20. Centrosome development in early mouse embryos as defined by an autoantibody against pericentriolar material.
    Cell. 1983 Dec;35(3 Pt 2):621-9 PMID: 6652679
  21. Cell cycle dynamics of an M-phase-specific cytoplasmic factor in Xenopus laevis oocytes and eggs.
    J Cell Biol. 1984 Apr;98(4):1247-55 PMID: 6425302
  22. Regulation of the cell cycle during early Xenopus development.
    Cell. 1984 Jul;37(3):731-42 PMID: 6378387
  23. Phosphoproteins are components of mitotic microtubule organizing centers.
    Proc Natl Acad Sci U S A. 1984 Jul;81(14):4439-43 PMID: 6379644
  24. Microtubule assembly nucleated by isolated centrosomes.
    Nature. 1984 Nov 15-21;312(5991):232-7 PMID: 6504137
  25. A polymer-dependent increase in phosphorylation of beta-tubulin accompanies differentiation of a mouse neuroblastoma cell line.
    J Cell Biol. 1985 Mar;100(3):764-74 PMID: 2857724
  26. An investigation of the centriole cycle using 3T3 and CHO cells.
    J Cell Sci. 1985 Oct;78:147-62 PMID: 4093469
  27. The events of the midblastula transition in Xenopus are regulated by changes in the cell cycle.
    Cell. 1987 Feb 13;48(3):399-407 PMID: 3802197
  28. Microtubule organizing centers.
    Annu Rev Cell Biol. 1985;1:145-72 PMID: 3916316
  29. Cyclic activation of histone H1 kinase during sea urchin egg mitotic divisions.
    Exp Cell Res. 1988 Jan;174(1):116-29 PMID: 2826194
  30. Relationship between nuclear DNA synthesis and centrosome reproduction in sea urchin eggs.
    J Exp Zool. 1987 Oct;244(1):89-100 PMID: 3694143
  31. Activation of multiple protein kinases during the burst in protein phosphorylation that precedes the first meiotic cell division in Xenopus oocytes.
    J Biol Chem. 1988 Feb 5;263(4):2009-19 PMID: 2448302
  32. Role of nuclear material in the early cell cycle of Xenopus embryos.
    Cell. 1988 Feb 26;52(4):525-33 PMID: 2830025
  33. Germinal vesicle components are not required for the cell-cycle oscillator of the early starfish embryo.
    Dev Biol. 1988 Jul;128(1):121-8 PMID: 3289983
  34. The Xenopus cdc2 protein is a component of MPF, a cytoplasmic regulator of mitosis.
    Cell. 1988 Jul 29;54(3):423-31 PMID: 3293802
  35. Purified maturation-promoting factor contains the product of a Xenopus homolog of the fission yeast cell cycle control gene cdc2+.
    Cell. 1988 Jul 29;54(3):433-9 PMID: 3293803
  36. Distribution of pericentriolar material in multipolar spindles induced by colcemid treatment in Chinese hamster ovary cells.
    J Cell Sci. 1988 Jan;89 ( Pt 1):57-65 PMID: 3047157
  37. cdc2 is a component of the M phase-specific histone H1 kinase: evidence for identity with MPF.
    Cell. 1988 Oct 21;55(2):371-8 PMID: 2844417
  38. Nuclear and cytoplasmic mitotic cycles continue in Drosophila embryos in which DNA synthesis is inhibited with aphidicolin.
    J Cell Biol. 1988 Dec;107(6 Pt 1):2009-19 PMID: 3143733
  39. Translation of cyclin mRNA is necessary for extracts of activated xenopus eggs to enter mitosis.
    Cell. 1989 Mar 24;56(6):947-56 PMID: 2564315
  40. Purification of MPF from starfish: identification as the H1 histone kinase p34cdc2 and a possible mechanism for its periodic activation.
    Cell. 1989 Apr 21;57(2):253-63 PMID: 2649251
  41. The cdc2 kinase is a nuclear protein that is essential for mitosis in mammalian cells.
    Cell. 1989 May 5;57(3):393-401 PMID: 2541912
  42. Cyclin synthesis drives the early embryonic cell cycle.
    Nature. 1989 May 25;339(6222):275-80 PMID: 2566917
Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.110.6.2033
Published
1990-06-00
Pages
2033-42
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2116137
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.110.6.2033 PMC Full Text View journal details
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