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PMID: 2186175 Published · ppublish English Journal Article

Human immunodeficiency virus-like, nonreplicating, gag-env particles assemble in a recombinant vaccinia virus expression system.

Journal of virology ·Vol. 64 ·No. 6 ·1990-06-00 ·Pages 2653-9

Haffar O, Garrigues J, Travis B, Moran P, Zarling J, Hu SL

Abstract

We report the assembly of human immunodeficiency virus (HIV)-like particles in African green monkey kidney cells coinfected with two recombinant vaccinia viruses, one carrying the HIV-1 gag and protease genes and the other the env gene. Biochemical analysis of particles sedimented from culture supernatants of doubly infected cells revealed that they were composed of gag proteins, primarily p24, as well as the env proteins gp120 and gp41. Thin-section immunoelectron microscopy showed that these particles were 100 to 120 nm in diameter, were characterized by the presence of cylindrical core structures, and displayed the mature gp120-gp41 complexes on their surfaces. Furthermore, thin-section immunoelectron microscopy analysis of infected cells showed that particle assembly and budding occurred at the plasma membrane. Nucleic acid hybridization suggested that the particles packaged only the gag mRNA but not the env mRNA. Therefore, the system we present is well suited for studies of HIV virion maturation. In addition, the HIV-like particles provide a novel and attractive approach for vaccine development.

MeSH Terms
Animals Antibodies, Monoclonal Cell Line Centrifugation, Density Gradient Gene Expression Gene Products, gag/genetics,isolation & purification,metabolism HIV/genetics,isolation & purification Nucleic Acid Hybridization RNA, Viral/genetics,isolation & purification Recombination, Genetic Vaccinia virus/genetics Viral Envelope Proteins/genetics,isolation & purification,metabolism
Chemicals
Antibodies, Monoclonal Gene Products, gag RNA, Viral Viral Envelope Proteins
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Haffar O
Department of Virology, Oncogen, Seattle, Washington 98121.
Garrigues J
Travis B
Moran P
Zarling J
Hu S L
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.64.6.2653-2659.1990
Published
1990-06-00
Pages
2653-9
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC249443
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.64.6.2653-2659.1990 PMC Full Text View journal details
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