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PMID: 21828278 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Review

Genome engineering with zinc-finger nucleases.

Genetics ·Vol. 188 ·No. 4 ·2011-08-00 ·Pages 773-82

Carroll D

Abstract

Zinc-finger nucleases (ZFNs) are targetable DNA cleavage reagents that have been adopted as gene-targeting tools. ZFN-induced double-strand breaks are subject to cellular DNA repair processes that lead to both targeted mutagenesis and targeted gene replacement at remarkably high frequencies. This article briefly reviews the history of ZFN development and summarizes applications that have been made to genome editing in many different organisms and situations. Considerable progress has been made in methods for deriving zinc-finger sets for new genomic targets, but approaches to design and selection are still being perfected. An issue that needs more attention is the extent to which available mechanisms of double-strand break repair limit the scope and utility of ZFN-initiated events. The bright prospects for future applications of ZFNs, including human gene therapy, are discussed.

MeSH Terms
Animals DNA Breaks, Double-Stranded Endonucleases/metabolism Gene Targeting Genetic Engineering Genome/genetics Humans Substrate Specificity Zinc Fingers/physiology
Chemicals
Endonucleases
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Carroll Dana
Department of Biochemistry, University of Utah School of Medicine, Salt Lake City, Utah 84112-5650, USA. dana@biochem.utah.edu
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Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
1943-2631
DOI
10.1534/genetics.111.131433
Email
dana@biochem.utah.edu
Published
2011-08-00
Pages
773-82
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC3176093
Subset
IM
Grants
NIGMS NIH HHS · R01 GM058504 · United States
NIGMS NIH HHS · R01 GM078571 · United States
NIGMS NIH HHS · GM078571 · United States
NIGMS NIH HHS · GM58504 · United States
External Links
PubMed source DOI: 10.1534/genetics.111.131433 PMC Full Text View journal details
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