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PMID: 218209 Published · ppublish English Journal Article

Synthesis and assembly of membrane glycoproteins: presence of leader peptide in nonglycosylated precursor of membrane glycoprotein of vesicular stomatitis virus.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 76 ·No. 2 ·1979-02-00 ·Pages 570-4

Irving RA, Toneguzzo F, Rhee SH, Hofmann T, Ghosh HP

Abstract

Translation of mRNA encoding vesicular stomatitis virus envelope glycoprotein G by as membrane-free ribosomal extract obtained from HeLa cells yielded a nonglycosylated protein (G1 (Mr 63,000). In the presence of added microsomal membranes, G1 was converted to the glycosylated protein (G2 (Mr 67,000) which is inserted in the membrane vesicles as a transmembrane protein. Labeling with methionine donated by wheat germ initiator tRNA1Met showed that G1 but not G2 contains methionine in the NH2-terminal position. Determination of the NH2-terminal sequence of G1, G2, and G showed that a leader peptide of 16 amino acids is present in G1 but absent from the glycosylated proteins G2 and G. This leader peptide contains at least 62% hydrophobic amino acids and is removed presumably during insertion of G1 into the membrane.

MeSH Terms
Amino Acid Sequence Glycoproteins/biosynthesis Membrane Proteins/biosynthesis Protein Biosynthesis Protein Precursors/metabolism RNA, Messenger/metabolism Solubility Vesicular stomatitis Indiana virus/metabolism Viral Proteins/biosynthesis
Chemicals
Glycoproteins Membrane Proteins Protein Precursors RNA, Messenger Viral Proteins
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Irving R A
Toneguzzo F
Rhee S H
Hofmann T
Ghosh H P
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32 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.76.2.570
Published
1979-02-00
Pages
570-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC382990
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.76.2.570 PMC Full Text View journal details
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