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PMID: 2176715 Published · ppublish English Journal Article

Site-directed mutagenesis of the Saccharomyces cerevisiae CDC25 gene: effects on mitotic growth and cAMP signalling.

Molecular & general genetics : MGG ·Vol. 223 ·No. 3 ·1990-09-00 ·Pages 426-32

Schomerus C, Munder T, Küntzel H

Abstract

A potential membrane-interacting site within the essential growth-controlling carboxy-terminal region of the CDC25 protein was interrupted by a lethal mutation (1461 Tyr----Asp and 1462 Leu----Arg). The elimination of two potential phosphorylation sites found in the same region (1489 Thr----Pro and 1584 Ser----Pro) does not affect growth but completely prevents glucose-induced cAMP signalling in the double mutant, whereas the single mutants produce normal or slightly retarded cAMP signals. A cluster of five potential targets for cAMP-dependent phosphorylation at the amino-terminal region could be deleted without affecting phenotypic properties. It is concluded that the carboxy-terminal 137 residues of the CDC25 protein are involved in three different functions: control of mitotic growth, glucose-induced hyperactivation of adenylate cyclase, and feed-back inhibition of cAMP synthesis.

Related Genes
CDC25
MeSH Terms
Amino Acid Sequence Base Sequence Cell Cycle Proteins Cloning, Molecular Cyclic AMP/metabolism Fungal Proteins/genetics,metabolism Genes, Fungal Mitosis Molecular Sequence Data Mutagenesis, Site-Directed Phosphorylation Restriction Mapping Saccharomyces cerevisiae/genetics,growth & development Signal Transduction/genetics ras-GRF1
Chemicals
Cell Cycle Proteins Fungal Proteins ras-GRF1 Cyclic AMP
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Schomerus C
Max-Planck-Institut für experimentelle Medizin, Abteilung Chemie, Göttingen, Federal Republic of Germany.
Munder T
Küntzel H
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00264449
Published
1990-09-00
Pages
426-32
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00264449
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