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PMID: 3043203 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A new RAS mutation that suppresses the CDC25 gene requirement for growth of Saccharomyces cerevisiae.

Molecular and cellular biology ·Vol. 8 ·No. 7 ·1988-07-00 ·Pages 2980-3

Camonis JH, Jacquet M

Abstract

In the yeast Saccharomyces cerevisiae, the activation of adenylate cyclase requires the products of the RAS genes and of CDC25. We isolated several dominant extragenic suppressors of the yeast cdc25 mutation. They did not suppress a thermosensitive allele of the adenylate cyclase gene (CDC35). One of these suppressors was a mutated RAS2 gene in which the transition C/G----T/A at position 455 resulted in replacement of threonine 152 by isoleucine in the protein. The same mutation in a v-Ha-ras gene reduces the affinity of p21 for guanine nucleotides (L.A. Feig, B. Pan, T.M. Roberts, and G.M. Cooper, Proc. Natl. Acad. Sci. USA 83:4607-4611, 1986). These results support a model in which the CDC25 gene product is the GDP-GTP exchange factor regulating the activity of the RAS gene product.

MeSH Terms

Adenylyl Cyclases/genetics,metabolism Gene Expression Regulation Genes, ras Mutation Saccharomyces cerevisiae/genetics,growth & development Suppression, Genetic

Chemicals

Adenylyl Cyclases

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Camonis J H

Groupe Information Génétique et Développement, Université de Paris-Sud, Orsay, France.

Jacquet M

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28 references, click to expand

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Article Info

Journal

Molecular and cellular biology

Abbr.

Mol Cell Biol

ISSN

0270-7306

DOI

10.1128/mcb.8.7.2980-2983.1988

Published

1988-07-00

Pages

2980-3

Language

English

Region

United States

NLM ID

8109087

PMCID

PMC363518

Subset

External Links

PubMed source DOI: 10.1128/mcb.8.7.2980-2983.1988 PMC Full Text View journal details

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A new RAS mutation that suppresses the CDC25 gene requirement for growth of Saccharomyces cerevisiae.

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