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PMID: 2174108 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A dominant negative mutation of the alpha retinoic acid receptor gene in a retinoic acid-nonresponsive embryonal carcinoma cell.

Molecular and cellular biology ·Vol. 10 ·No. 12 ·1990-12-00 ·Pages 6445-53

Pratt MA, Kralova J, McBurney MW

Abstract

Pluripotential embryonal carcinoma cells such as those of the P19 line differentiate when exposed to retinoic acid (RA). The RAC65 cell line is a mutant clone of P19 cells selected to be RA nonresponsive. RAC65 cells carry a rearrangement affecting one of the genes encoding a nuclear retinoic acid receptor (RAR alpha). The mutant gene encodes a protein, RAR alpha', that has lost its 70 C-terminal amino acids, thus truncating the RA-binding domain. The RAR alpha' was found to be a dominant repressor of transcription from an RA-responsive target gene; however, expression of RAR alpha' was insufficient to confer RA nonresponsiveness, suggesting that RAC65 cells carry an additional mutation(s) affecting RA-induced genes.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Blotting, Southern Carrier Proteins/genetics Cell Differentiation Cell Line DNA, Neoplasm/genetics Drug Resistance Gene Library Genes Genes, Dominant Mice Molecular Sequence Data Mutation RNA, Messenger/genetics Receptors, Retinoic Acid Teratoma/genetics Tretinoin/metabolism,pharmacology
Chemicals
Carrier Proteins DNA, Neoplasm RNA, Messenger Receptors, Retinoic Acid Tretinoin
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Pratt M A
Department of Medicine, University of Ottawa, Ontario, Canada.
Kralova J
McBurney M W
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.10.12.6445-6453.1990
Published
1990-12-00
Pages
6445-53
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC362921
Subset
IM
Databases
GENBANK
M60909
External Links
PubMed source DOI: 10.1128/mcb.10.12.6445-6453.1990 PMC Full Text View journal details
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