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PMID: 2163395 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Transposition in Shigella dysenteriae: isolation and analysis of IS911, a new member of the IS3 group of insertion sequences.

Journal of bacteriology ·Vol. 172 ·No. 7 ·1990-07-00 ·Pages 4090-9

Prère MF, Chandler M, Fayet O

Abstract

Twenty-nine clear-plaque mutants of bacteriophage lambda were isolated from a Shigella dysenteriae lysogen. Three were associated with insertions in the cI gene: two were due to insertion of IS600, and the third resulted from insertion of a new element, IS911. IS911 is 1,250 base pairs (bp) long, carries 27-bp imperfect terminal inverted repeats, and generates 3-bp duplications of the target DNA on insertion. It was found in various copy numbers in all four species of Shigella tested and in Escherichia coli K-12 but not in E. coli W. Analysis of IS911-mediated cointegrate molecules indicated that the majority were generated without duplication of IS911. They appeared to result from direct insertion via one end of the element and the neighboring region of DNA, which resembles a terminal inverted repeat of IS911. Nucleotide sequence analysis revealed that IS911 carries two consecutive open reading frames which code for potential proteins showing similarities to those of the IS3 group of elements.

MeSH Terms
Amino Acid Sequence Base Sequence Blotting, Southern DNA Transposable Elements DNA, Bacterial/genetics,isolation & purification Escherichia coli/genetics Molecular Sequence Data Nucleic Acid Hybridization Oligonucleotide Probes Plasmids Restriction Mapping Sequence Homology, Nucleic Acid Shigella/genetics Shigella dysenteriae/genetics Species Specificity
Chemicals
DNA Transposable Elements DNA, Bacterial Oligonucleotide Probes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Prère M F
Centre de Biochimie et Génétique Cellulaires du Centre National de la Recherche Scientifique, Toulouse, France.
Chandler M
Fayet O
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.172.7.4090-4099.1990
Published
1990-07-00
Pages
4090-9
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC213396
Subset
IM
Databases
GENBANK
X17613
External Links
PubMed source DOI: 10.1128/jb.172.7.4090-4099.1990 PMC Full Text View journal details
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