Gross rearrangements within the 5'-untranslated region of the picornaviral genomes.

Nucleic acids research ·Vol. 18 ·No. 11 ·1990-06-11 ·Pages 3371-5

Pilipenko EV, Blinov VM, Agol VI

Abstract

An analysis of reported nucleotide sequences revealed several cases of gross rearrangements in the 5'-untranslated region (5-UTR) of picornaviral genomes. A large (greater than 100 nt) duplication was discovered in a downstream region of poliovirus 5-UTR involved in the translational control. Properties of the poliovirus mutants with large deletions [Kuge and Nomoto (1987) J. Virol. 61, 1478-1487] show that a single copy of the appropriate repeating unit is compatible with a wild type phenotype of the virus. In contrast to poliovirus and another enterovirus genomes, human rhinovirus RNAs contain only a single copy of this repeating unit. Another similarly large repeat was found in an upstream segment of the bovine enterovirus 5-UTR. A comparison of the primary and secondary structures of cardio- and aphthovirus 5-UTRs demonstrated the existence of a large (ca. 250 nucleotides) insertion/deletion in a region preceding the poly(C) tract. The two latter rearrangements appear to involve elements of the viral genome replication machinery. Possible origin as well as evolutionary and functional implications of these structural peculiarities are discussed.

MeSH Terms

Aphthovirus/genetics Base Sequence Encephalomyocarditis virus/genetics Enterovirus/genetics Gene Rearrangement Genes, Viral Introns Molecular Sequence Data Mutation Nucleic Acid Conformation Picornaviridae/genetics Poliovirus/genetics Repetitive Sequences, Nucleic Acid Sequence Homology, Nucleic Acid

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Pilipenko E V

Institute of Poliomyelitis and Viral Encephalitides, USSR Academy of Medical Sciences, Moscow Region.

Blinov V M

Agol V I

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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/18.11.3371

Published

1990-06-11

Pages

3371-5

Language

English

Region

England

NLM ID

0411011

PMCID

PMC330946

Subset

External Links

PubMed source DOI: 10.1093/nar/18.11.3371 PMC Full Text View journal details

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Gross rearrangements within the 5'-untranslated region of the picornaviral genomes.

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