Home LiteratureArticle Details
PMID: 2161946 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Resistance to influenza virus and vesicular stomatitis virus conferred by expression of human MxA protein.

Journal of virology ·Vol. 64 ·No. 7 ·1990-07-00 ·Pages 3370-5

Pavlovic J, Zürcher T, Haller O, Staeheli P

Abstract

MxA and MxB are interferon-induced proteins of human cells and are related to the murine protein Mx1, which confers selective resistance to influenza virus. In contrast to the nuclear murine protein Mx1, MxA and MxB are located in the cytoplasm, and their role in the interferon-induced antiviral state was unknown. In this report we show that transfected cell lines expressing MxA acquired a high degree of resistance to influenza A virus. Surprisingly, MxA also conferred resistance to vesicular stomatitis virus. Expression of MxA in transfected 3T3 cells had no effect on the multiplication of two picornaviruses, a togavirus, or herpes simplex virus type 1. Treatment of MxA-expressing cells with antibodies to mouse alpha-beta interferon did not abolish the resistance phenotype. The conclusion that resistance to influenza virus and vesicular stomatitis virus was due to the specific action of MxA is further supported by the observation that transfected 3T3 cell lines expressing the related MxB failed to acquire virus resistance.

MeSH Terms
Animals Blotting, Western Cell Compartmentation Cell Line GTP-Binding Proteins Gene Expression Immunity, Innate In Vitro Techniques Interferon Type I/physiology Mice Myxovirus Resistance Proteins Orthomyxoviridae/growth & development Picornaviridae/growth & development Proteins/physiology Simplexvirus/growth & development Togaviridae/growth & development Transfection Vesicular stomatitis Indiana virus/growth & development Viral Plaque Assay Virus Replication
Chemicals
Interferon Type I MX1 protein, human MX2 protein, human Mx1 protein, mouse Myxovirus Resistance Proteins Proteins GTP-Binding Proteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Pavlovic J
Institut für Immunologie und Virologie, Universität Zürich, Switzerland.
Zürcher T
Haller O
Staeheli P
References (31)
31 references, click to expand
  1. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  2. Interferon-induced proteins and the antiviral state.
    Adv Virus Res. 1990;38:147-200 PMID: 1699395
  3. Preliminary characterization of the particles from productive and abortive infections of L cells by fowl plague virus.
    Ann Microbiol (Paris). 1979 Jul;130B(1):85-100 PMID: 507639
  4. Transformation of mammalian cells to antibiotic resistance with a bacterial gene under control of the SV40 early region promoter.
    J Mol Appl Genet. 1982;1(4):327-41 PMID: 6286831
  5. Mechanism of interferon action: inhibition of vesicular stomatitis virus replication in human amnion U cells by cloned human leukocyte interferon. II. Effect on viral macromolecular synthesis.
    J Biol Chem. 1983 Oct 10;258(19):12026-33 PMID: 6311835
  6. Combined action of mouse alpha and beta interferons in influenza virus-infected macrophages carrying the resistance gene Mx.
    J Virol. 1984 Mar;49(3):709-16 PMID: 6321758
  7. Interferon-induced protein Mx accumulates in nuclei of mouse cells expressing resistance to influenza viruses.
    Virology. 1985 Jan 15;140(1):192-6 PMID: 3880978
  8. Polyclonal and monoclonal antibodies to the interferon-inducible protein Mx of influenza virus-resistant mice.
    J Biol Chem. 1985 Feb 10;260(3):1821-5 PMID: 3881442
  9. Inhibition of influenza viral mRNA synthesis in cells expressing the interferon-induced Mx gene product.
    J Virol. 1985 Oct;56(1):201-6 PMID: 2411949
  10. Topology of signal recognition particle receptor in endoplasmic reticulum membrane.
    Nature. 1985 Nov 28-Dec 4;318(6044):334-8 PMID: 2999608
  11. Mx protein: constitutive expression in 3T3 cells transformed with cloned Mx cDNA confers selective resistance to influenza virus.
    Cell. 1986 Jan 17;44(1):147-58 PMID: 3000619
  12. Adenovirus VAI RNA antagonizes the antiviral action of interferon by preventing activation of the interferon-induced eIF-2 alpha kinase.
    Cell. 1986 Apr 25;45(2):195-200 PMID: 3698097
  13. Interferon-induced human protein with homology to protein Mx of influenza virus-resistant mice.
    Mol Cell Biol. 1985 Aug;5(8):2150-3 PMID: 3939324
  14. Inhibition of vesicular stomatitis viral mRNA synthesis by interferons.
    J Virol. 1987 Mar;61(3):653-60 PMID: 3027394
  15. GTP-binding domain: three consensus sequence elements with distinct spacing.
    Proc Natl Acad Sci U S A. 1987 Apr;84(7):1814-8 PMID: 3104905
  16. IFN-alpha induced human 78 kD protein: purification and homologies with the mouse Mx protein, production of monoclonal antibodies, and potentiation effect of IFN-gamma.
    J Interferon Res. 1987 Aug;7(4):331-43 PMID: 3117906
  17. Interferon-induced Mx protein: a mediator of cellular resistance to influenza virus.
    Interferon. 1987;8:1-23 PMID: 2445687
  18. Transport of the murine Mx protein into the nucleus is dependent on a basic carboxy-terminal sequence.
    J Interferon Res. 1987 Oct;7(5):657-69 PMID: 2445861
  19. Constitutive expression of (2'-5') oligo A synthetase confers resistance to picornavirus infection.
    Nature. 1987 Dec 10-16;330(6148):587-8 PMID: 2825034
  20. A family of interferon-induced Mx-related mRNAs encodes cytoplasmic and nuclear proteins in rat cells.
    J Virol. 1988 Jul;62(7):2386-93 PMID: 3373571
  21. Antiviral state against influenza virus neutralized by microinjection of antibodies to interferon-induced Mx proteins.
    EMBO J. 1988 May;7(5):1315-20 PMID: 3409866
  22. Influenza virus-susceptible mice carry Mx genes with a large deletion or a nonsense mutation.
    Mol Cell Biol. 1988 Oct;8(10):4518-23 PMID: 2903437
  23. Mechanisms of the antiviral action of interferons.
    Prog Nucleic Acid Res Mol Biol. 1988;35:27-72 PMID: 2464840
  24. The signal recognition particle receptor mediates the GTP-dependent displacement of SRP from the signal sequence of the nascent polypeptide.
    Cell. 1989 May 19;57(4):599-610 PMID: 2541918
  25. Homology of 54K protein of signal-recognition particle, docking protein and two E. coli proteins with putative GTP-binding domains.
    Nature. 1989 Aug 10;340(6233):478-82 PMID: 2502717
  26. Model for signal sequence recognition from amino-acid sequence of 54K subunit of signal recognition particle.
    Nature. 1989 Aug 10;340(6233):482-6 PMID: 2502718
  27. A ubiquitous mammalian expression vector, pHMG, based on a housekeeping gene promoter.
    Nucleic Acids Res. 1989 Oct 25;17(20):8389 PMID: 2813075
  28. cDNA structures and regulation of two interferon-induced human Mx proteins.
    Mol Cell Biol. 1989 Nov;9(11):5062-72 PMID: 2481229
  29. Purification and characterization of a human Mx protein.
    J Interferon Res. 1989 Dec;9(6):679-89 PMID: 2607176
  30. Cloning and sequence analyses of cDNAs for interferon- and virus-induced human Mx proteins reveal that they contain putative guanine nucleotide-binding sites: functional study of the corresponding gene promoter.
    J Virol. 1990 Mar;64(3):1171-81 PMID: 2154602
  31. Role of interferon in the pathogenesis of virus diseases in mice as demonstrated by the use of anti-interferon serum. I. Rapid evolution of encephalomyocarditis virus infection.
    J Exp Med. 1976 Nov 2;144(5):1305-15 PMID: 186554
Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.64.7.3370-3375.1990
Published
1990-07-00
Pages
3370-5
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC249583
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.64.7.3370-3375.1990 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2