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PMID: 3373571 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A family of interferon-induced Mx-related mRNAs encodes cytoplasmic and nuclear proteins in rat cells.

Journal of virology ·Vol. 62 ·No. 7 ·1988-07-00 ·Pages 2386-93

Meier E, Fäh J, Grob MS, End R, Staeheli P, Haller O

Abstract

Mouse Mx protein, an interferon (IFN)-induced nuclear protein, confers selective resistance to influenza virus. We show here that, as with influenza virus-resistant Mx+ mouse embryo cells, influenza virus mRNA accumulation and protein synthesis are strongly inhibited in rat embryo cells treated with IFN-alpha/beta. IFN-alpha/beta induced in rat cells the synthesis of Mx-related mRNAs migrating on Northern (RNA) gels as two bands of about 3.5 and 2.5 kilobases which directed the synthesis of three electrophoretically distinct proteins called rat Mx proteins 1, 2, and 3. The three rat proteins were antigenically related to the mouse Mx protein but differed in molecular weight and intracellular location. Rat Mx protein 1 was found predominantly in the nucleus and, on the basis of several criteria, resembled the nuclear mouse Mx protein. It was induced by IFN-alpha/beta in all 28 inbred rat strains tested. Rat Mx proteins 2 and 3 differed from protein 1 at the carboxy terminus and were predominantly cytoplasmic like the human Mx homolog. Sequence data of partial cDNA clones indicate that three Mx-related genes, rather than one, exist in the rat.

MeSH Terms
Animals Cell Nucleus/analysis Cells, Cultured Cytoplasm/analysis DNA/genetics Fibroblasts/drug effects,ultrastructure GTP-Binding Proteins Gene Expression Regulation/drug effects Influenza A virus/physiology Interferon Type I/pharmacology Macrophages/drug effects,ultrastructure Myxovirus Resistance Proteins Proteins/genetics RNA, Messenger/genetics Rats Rats, Inbred Strains Stimulation, Chemical Virus Replication
Chemicals
Interferon Type I Mx1 protein, mouse Myxovirus Resistance Proteins Proteins RNA, Messenger DNA GTP-Binding Proteins
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Meier E
Institute for Immunology and Virology, University of Zurich, Switzerland.
Fäh J
Grob M S
End R
Staeheli P
Haller O
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.62.7.2386-2393.1988
Published
1988-07-00
Pages
2386-93
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC253396
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.62.7.2386-2393.1988 PMC Full Text View journal details
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