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PMID: 2159556 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Intrachromosomal recombination mediated by papovavirus large T antigens.

Journal of virology ·Vol. 64 ·No. 6 ·1990-06-00 ·Pages 2958-66

St-Onge L, Bouchard L, Laurent S, Bastin M

Abstract

To investigate the mechanism by which the large T antigen (T-Ag) of polyomavirus and simian virus 40 can promote recombination in mammalian cells, we analyzed homologous recombination events occurring between two defective copies of the polyomavirus middle T (pmt) oncogene lying in close proximity on the same chromosome in a rat cell line. Reconstitution of a functional pmt gene by spontaneous recombination occurred at a rate of about 2 x 10(-7) per cell generation. Introduction of the polyomavirus large T (plt) oncogene into the cell line by DNA transfection promoted recombination very efficiently, with rates in the range of 10(-1) to 10(-2) per cell generation. Recombination was independent of any amplification of viral sequences and could even be promoted by the large T-Ag from simian virus 40, which cannot activate polyomavirus DNA replication. To explain the role of large T-Ag, we propose a novel mechanism of nonconservative recombination involving slipped-strand mispairing between the two viral repeats followed by gap repair synthesis.

MeSH Terms
Animals Antigens, Polyomavirus Transforming/genetics Blotting, Southern Cell Line Chromosomes/physiology DNA Replication DNA Transposable Elements Defective Viruses/genetics Mutation Oncogenes Plasmids Polyomavirus/genetics,immunology Promoter Regions, Genetic Recombination, Genetic Restriction Mapping Simian virus 40/genetics,immunology
Chemicals
Antigens, Polyomavirus Transforming DNA Transposable Elements
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
St-Onge L
Department of Microbiology, University of Sherbrooke, Quebec, Canada.
Bouchard L
Laurent S
Bastin M
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54 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.64.6.2958-2966.1990
Published
1990-06-00
Pages
2958-66
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC249480
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.64.6.2958-2966.1990 PMC Full Text View journal details
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