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PMID: 2159550 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Mutational analysis of simian virus 40 small-t antigen.

Journal of virology ·Vol. 64 ·No. 6 ·1990-06-00 ·Pages 2895-900

Jog P, Joshi B, Dhamankar V, Imperiale MJ, Rutila J, Rundell K

Abstract

Several point mutations in the simian virus 40 (SV40) small-t antigen have been analyzed for their effects on protein stability, transformation, transactivation, and binding of two cellular proteins. All mutations which affected cysteine residues in two cysteine clusters produced highly unstable small-t antigens. Four point mutations outside these clusters and one in-frame deletion mutant, dl890, produced stable proteins but reduced transformation efficiency. These were able to transactivate the EII promoter and bind the cellular proteins, suggesting that these activities are not sufficient for small-t-mediated enhancement of transformation.

MeSH Terms
Amino Acid Sequence Animals Antigens, Polyomavirus Transforming/genetics Cell Line Cell Transformation, Viral Chromosome Deletion DNA Replication/drug effects Molecular Sequence Data Mutation Plasmids Simian virus 40/genetics,immunology Theophylline/pharmacology Transcriptional Activation
Chemicals
Antigens, Polyomavirus Transforming Theophylline
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Jog P
Department of Microbiology and Immunology, Northwestern University Medical School, Chicago, Illinois 60611-3008.
Joshi B
Dhamankar V
Imperiale M J
Rutila J
Rundell K
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.64.6.2895-2900.1990
Published
1990-06-00
Pages
2895-900
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC249472
Subset
IM
Grants
NCI NIH HHS · CA19816 · United States
NCI NIH HHS · CA21327 · United States
External Links
PubMed source DOI: 10.1128/JVI.64.6.2895-2900.1990 PMC Full Text View journal details
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